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Three chiral stable free radicals containing a hydroxymethyl group have been resolved by biocatalysis. By recrystallization and/or re-esterification each molecule was produced in very high enantiomeric purity. The resolved 1-oxyl-3-hydroxymethyl-2,2,5,5-tetramethylpyrrolidine alcohols were converted to methanethiosulfonate spin labels, 1-oxyl-3-methanesulfonylthiomethyl-2,2,5,5-tetramethylpyrrolidines...
A pyrroline nitroxide based cyclic tetrasubstituted α-amino acid and paramagnetic homoproline and their derivatives are described. Introduction of a new paramagnetic protecting group to follow the incorporation of an amino acid into peptides by EPR is also suggested.
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