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Background To gain insight into what differences might restrict the capacity for limb regeneration in Xenopus froglets, we used High Performance Liquid Chromatography (HPLC)/double mass spectrometry to characterize protein expression during fibroblastema formation in the amputated froglet hindlimb, and compared the results to those obtained previously for blastema formation in the axolotl limb. ...
We used an antibody array to compare the protein expression of matrix metalloproteinases (MMPs)‐1, ‐2, ‐3, ‐8, ‐9, ‐10, and ‐13, as well as the tissue inhibitors of metalloproteinases (TIMPs)‐1, ‐2, and ‐4 during blastema formation in amputated hindlimbs of regeneration‐competent wild‐type axolotls and stage‐54 Xenopus, and regeneration‐deficient short‐toes axolotls and Xenopus froglets. Expression...
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