Vasilis P. Androutsopoulos

article

Physiological and anthocyanin biosynthesis genes response induced by vanadium stress in mustard genotypes with distinct photosynthetic activity

Muhammad Imtiaz, Muhammad Adnan Mushtaq, Muhammad Amjad Nawaz, Muhammad Ashraf, more

Environmental Toxicology and Pharmacology > 2018 > 62 > C > 20-29

The present study aimed to elucidate the photosynthetic performance, antioxidant enzyme activities, anthocyanin contents, anthocyanin biosynthetic gene expression, and vanadium uptake in mustard genotypes (purple and green) that differ in photosynthetic capacity under vanadium stress. The results indicated that vanadium significantly reduced photosynthetic activity in both genotypes. The activities...

article

Significant metabolic improvement by a water extract of olives: animal and human evidence

Nikolaos Peroulis, Vasilis P. Androutsopoulos, George Notas, Stella Koinaki, more

European Journal of Nutrition > 2019 > 58 > 6 > 2545-2560

Purpose Dyslipidemia and impaired glucose metabolism are the main health issues of growing prevalence and significant high healthcare cost, requiring novel prevention and/or therapeutic approaches. Epidemiological and animal studies revealed that olive oil is an important dietary constituent, inducing normolipidemia. However, no studies have specifically investigated the polyphenol-rich water extract...

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Tangeretin inhibits the proliferation of human breast cancer cells via CYP1A1/CYP1B1 enzyme induction and CYP1A1/CYP1B1–mediated metabolism to the product 4′ hydroxy tangeretin

Somchaiya Surichan, Randolph R. Arroo, Aristidis M. Tsatsakis, Vasilis P. Androutsopoulos

Toxicology in Vitro > 2018 > 50 > C > 274-284

Tangeretin is a polymethoxylated flavone with multifaceted anticancer activity. In the present study, the metabolism of tangeretin was evaluated in the CYP1 expressing human breast cancer cell lines MCF7 and MDA–MB–468 and in the normal breast cell line MCF10A. Tangeretin was converted to 4′ OH tangeretin by recombinant CYP1 enzymes and by CYP1 enzymes expressed in MCF7 and MDA–MB–468 cells. This...

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Cytochrome P450 CYP1 metabolism of hydroxylated flavones and flavonols: Selective bioactivation of luteolin in breast cancer cells

Nicola E. Wilsher, Randolph R. Arroo, Minos–Timotheos Matsoukas, Aristidis M. Tsatsakis, more

Food and Chemical Toxicology > 2017 > 110 > C > 383-394

Natural flavonoids with methoxy substitutions are metabolized by CYP1 enzymes to yield the corresponding demethylated products. The present study aimed to characterize the metabolism and further antiproliferative activity of the hydroxylated flavonoids apigenin, luteolin, scutellarein, kaempferol and quercetin in CYP1 recombinant enzymes and in the CYP1 expressing cell lines MCF7 and MDA–MB–468, respectively...

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Human exposure to endocrine disrupting chemicals: effects on the male and female reproductive systems

Stavros Sifakis, Vasilis P. Androutsopoulos, Aristeidis M. Tsatsakis, Demetrios A. Spandidos

Environmental Toxicology and Pharmacology > 2017 > 51 > C > 56-70

Endocrine disrupting chemicals (EDCs) comprise a group of chemical compounds that have been examined extensively due to the potential harmful effects in the health of human populations. During the past decades, particular focus has been given to the harmful effects of EDCs to the reproductive system. The estimation of human exposure to EDCs can be broadly categorized into occupational and environmental...

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Natural products—friends or foes?

Denisa Margină, Mihaela Ilie, Daniela Grădinaru, Vasilis P. Androutsopoulos, more

Toxicology Letters > 2015 > 236 > 3 > 154-167

A trend in the general population has been observed in recent years regarding the orientation toward preventive measures in health; in this context the increased interest from the users and researchers concerning the active effect of food supplements on the health state and on longevity, is noticeable. All over the world, the consumption of natural foods and of vegetal supplements has increased spectacularly...

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Benzo[a]pyrene sensitizes MCF7 breast cancer cells to induction of G1 arrest by the natural flavonoid eupatorin-5-methyl ether, via activation of cell signaling proteins and CYP1-mediated metabolism

Vasilis P. Androutsopoulos, Aristeidis M. Tsatsakis

Toxicology Letters > 2014 > 230 > 2 > 304-313

Eupatorin-5-methyl ether (E5M) is a flavone containing 4 methoxy groups that is present in plants with medicinal activity, whereas luteolin (L) is a polyhydroxylated flavone commonly encountered in dietary products. In the present study we investigated the interaction of the two flavonoids with cytochrome P450 CYP1 enzymes in breast cancer MCF7 cells. Both compounds induced a dose dependent increase...

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Genetic diversity of the KCNE1 gene and susceptibility to postoperative atrial fibrillation

Konstantinos V. Voudris, Stavros Apostolakis, Panagiotis Karyofillis, Konstantinos Doukas, more

American Heart Journal > 2014 > 167 > 2 > 274-280.e1

The human KCNE1 protein forms the β-subunit of the IKs potassium channel and is important in the regulation of the atrial action potential duration. The purpose of this study was to investigate the association between the nonsynonymous 112G>A mutation of the KCNE1 gene and postcardiac surgery atrial fibrillation (AF).A cohort of patients scheduled for cardiac surgery was prospectively recruited...

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Expression profile of CYP1A1 and CYP1B1 enzymes in endometrial tumors

Ioannis Spyrou, Stavros Sifakis, Achilles Ploumidis, Alexandros E. Papalampros, more

Tumor Biology > 2014 > 35 > 10 > 9549-9556

The cytochrome P450 CYP1A1 and CYP1B1 enzymes are phase I extrahepatic enzymes involved in the activation of pro-carcinogenic compounds to carcinogenic metabolites. Although differential overexpression of CYP1A1 and CYP1B1 has been documented at the messenger RNA (mRNA) and protein level, studies that have examined CYP1 expression by enzyme activity assays are limited. In the current study, the expression...

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Phytoestrogens as natural prodrugs in cancer prevention: towards a mechanistic model

Randolph R. J. Arroo, Kenneth Beresford, Avninder S. Bhambra, Mike Boarder, more

Phytochemistry Reviews > 2014 > 13 > 4 > 853-866

It has been widely acknowledged that regular consumption of fresh fruits and vegetables is linked with a relatively low incidence of cancers (e.g. breast, cervix, and colon). Notably, dietary polyphenolic compounds that show some structural similarity to human estrogen, e.g. isoflavones, coumestans, lignans, flavones, have been proposed to play a role in cancer prevention. However, at present there...

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A mechanistic overview of health associated effects of low levels of organochlorine and organophosphorous pesticides

Vasilis P. Androutsopoulos, Antonio F. Hernandez, Jyrki Liesivuori, Aristidis M. Tsatsakis

Toxicology > 2013 > 307 > Complete > 89-94

Organochlorine and organophosphate pesticides are compounds that can be detected in human populations as a result of occupational or residential exposure. Despite their occurrence in considerably low levels in humans, their biological effects are hazardous since they interact with a plethora of enzymes, proteins, receptors and transcription factors. In this review we summarize the cell and molecular...

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Pesticides and oncogenic modulation

Elena Vakonaki, Vasilis P. Androutsopoulos, Jyrki Liesivuori, Aristidis M. Tsatsakis, more

Toxicology > 2013 > 307 > Complete > 42-45

Pesticides constitute a diverse class of chemicals used for the protection of agricultural products. Several lines of evidence demonstrate that organochlorine and organophosphate pesticides can cause malignant transformation of cells in in vitro and in vivo models. In the current minireview a comprehensive summary of recent in vitro findings is presented along with data reported from human population...

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The flavonoids diosmetin and luteolin exert synergistic cytostatic effects in human hepatoma HepG2 cells via CYP1A-catalyzed metabolism, activation of JNK and ERK and P53/P21 up-regulation

Vasilis P. Androutsopoulos, Demetrios A. Spandidos

The Journal of Nutritional Biochemistry > 2013 > 24 > 2 > 496-504

Various types of tumors are known to overexpress enzymes belonging to the CYP1 family of cytochromes P450. The present study aimed to characterize the metabolism and further antiproliferative activity of the natural flavonoid diosmetin in the CYP1-expressing human hepatoma cell line HepG2. Diosmetin was converted to luteolin in HepG2 cells after 12 and 30 h of incubation. In the presence of the CYP1A...

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Bioactivation of the citrus flavonoid nobiletin by CYP1 enzymes in MCF7 breast adenocarcinoma cells

Somchaiya Surichan, Vasilis P. Androutsopoulos, Stavros Sifakis, Eleni Koutala, more

Food and Chemical Toxicology > 2012 > 50 > 9 > 3320-3328

Recent studies have demonstrated cytochrome P450 CYP1-mediated metabolism and CYP1-enzyme induction by naturally occurring flavonoids in cancer cell line models. The arising metabolites often exhibit higher activity than the parent compound. In the present study we investigated the CYP1-mediated metabolism of the citrus polymethoxyflavone nobiletin by recombinant CYP1 enzymes and MCF7 breast adenocarcinoma...

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Role of paraoxonase 1 (PON1) in organophosphate metabolism: Implications in neurodegenerative diseases

Vasilis P. Androutsopoulos, Konstantinos Kanavouras, Aristidis M. Tsatsakis

Toxicology and Applied Pharmacology > 2011 > 256 > 3 > 418-424

Organophosphate pesticides are a class of compounds that are widely used in agricultural and rural areas. Paraoxonase 1 (PON1) is a phase-I enzyme that is involved in the hydrolysis of organophosphate esters. Environmental poisoning by organophosphate compounds has been the main driving force of previous research on PON1 enzymes. Recent discoveries in animal models have revealed the important role...

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Anticancer effects of the metabolic products of the resveratrol analogue, DMU-212: Structural requirements for potency

Vasilis P. Androutsopoulos, Ketan C. Ruparelia, Athanasios Papakyriakou, Harilaos Filippakis, more

European Journal of Medicinal Chemistry > 2011 > 46 > 6 > 2586-2595

The methoxylated trans-stilbene resveratrol analogue, (E)-3,4,5,4′-tetramethoxystilbene (1), has shown promising antiproliferative activity in in vitro cell line and in vivo models. In vivo 1 gives rise to several metabolic products through demethylation or hydroxylation reactions at the stilbene moiety. In the present study we examined the anticancer activity of 1 and the metabolites (E)-3′-hydroxy-3,4,5,4′-tetramethoxystilbene...

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Comparative CYP1A1 and CYP1B1 substrate and inhibitor profile of dietary flavonoids

Vasilis P. Androutsopoulos, Athanasios Papakyriakou, Dionisios Vourloumis, Demetrios A. Spandidos

Bioorganic & Medicinal Chemistry > 2011 > 19 > 9 > 2842-2849

CYP1A1 and CYP1B1 are two extrahepatic enzymes that have been implicated in carcinogenesis and cancer progression. Selective inhibition of CYP1A1 and CYP1B1 by dietary constituents, notably the class of flavonoids, is a widely accepted paradigm that supports the concept of dietary chemoprevention. In parallel, recent studies have documented the ability of CYP1 enzymes to selectively metabolize dietary...

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Dietary flavonoids in cancer therapy and prevention: Substrates and inhibitors of cytochrome P450 CYP1 enzymes

Vasilis P. Androutsopoulos, Athanasios Papakyriakou, Dionisios Vourloumis, Aristidis M. Tsatsakis, more

Pharmacology and Therapeutics > 2010 > 126 > 1 > 9-20

Flavonoids are polyphenolic compounds that have attracted the attention of the scientific community as the hallmark molecules responsible for cancer prevention by a plethora of different mechanisms. One of their most important characteristics, responsible for their cancer preventive properties, is their interaction with cytochrome P450 CYP1 enzymes. Flavonoids have traditionally been described as...

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CYP1-mediated antiproliferative activity of dietary flavonoids in MDA-MB-468 breast cancer cells

Vasilis P. Androutsopoulos, Ketan Ruparelia, Randolph R.J. Arroo, Aristidis M. Tsatsakis, more

Toxicology > 2009 > 264 > 3 > 162-170

Among the different mechanisms proposed to explain the cancer-protecting effect of dietary flavonoids, substrate-like interactions with cytochrome P450 CYP1 enzymes have recently been explored. In the present study, the metabolism of the flavonoids chrysin, baicalein, scutellarein, sinensetin and genkwanin by recombinant CYP1A1, CYP1B1 and CYP1A2 enzymes, as well as their antiproliferative activity...

article

The Methoxylated Flavones Eupatorin and Cirsiliol Induce CYP1 Enzyme Expression in MCF7 Cells

Vasilis P. Androutsopoulos, Naichang Li, Randolph R. J. Arroo

Journal of Natural Products > 2009 > 72 > 8 > 1390 - 1394

INFONA - science communication portal

Search results for: Vasilis P. Androutsopoulos

Physiological and anthocyanin biosynthesis genes response induced by vanadium stress in mustard genotypes with distinct photosynthetic activity

Significant metabolic improvement by a water extract of olives: animal and human evidence

Tangeretin inhibits the proliferation of human breast cancer cells via CYP1A1/CYP1B1 enzyme induction and CYP1A1/CYP1B1–mediated metabolism to the product 4′ hydroxy tangeretin

Cytochrome P450 CYP1 metabolism of hydroxylated flavones and flavonols: Selective bioactivation of luteolin in breast cancer cells

Human exposure to endocrine disrupting chemicals: effects on the male and female reproductive systems

Natural products—friends or foes?

Benzo[a]pyrene sensitizes MCF7 breast cancer cells to induction of G1 arrest by the natural flavonoid eupatorin-5-methyl ether, via activation of cell signaling proteins and CYP1-mediated metabolism

Genetic diversity of the KCNE1 gene and susceptibility to postoperative atrial fibrillation

Expression profile of CYP1A1 and CYP1B1 enzymes in endometrial tumors

Phytoestrogens as natural prodrugs in cancer prevention: towards a mechanistic model

A mechanistic overview of health associated effects of low levels of organochlorine and organophosphorous pesticides

Pesticides and oncogenic modulation

The flavonoids diosmetin and luteolin exert synergistic cytostatic effects in human hepatoma HepG2 cells via CYP1A-catalyzed metabolism, activation of JNK and ERK and P53/P21 up-regulation

Bioactivation of the citrus flavonoid nobiletin by CYP1 enzymes in MCF7 breast adenocarcinoma cells

Role of paraoxonase 1 (PON1) in organophosphate metabolism: Implications in neurodegenerative diseases

Anticancer effects of the metabolic products of the resveratrol analogue, DMU-212: Structural requirements for potency

Comparative CYP1A1 and CYP1B1 substrate and inhibitor profile of dietary flavonoids

Dietary flavonoids in cancer therapy and prevention: Substrates and inhibitors of cytochrome P450 CYP1 enzymes

CYP1-mediated antiproliferative activity of dietary flavonoids in MDA-MB-468 breast cancer cells

The Methoxylated Flavones Eupatorin and Cirsiliol Induce CYP1 Enzyme Expression in MCF7 Cells

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