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C1 inhibitor was identified in human brain tissue by Western blotting and by immunohistochemistry using multiple antibodies to the native protein. The presence of C1 inhibitor mRNA was identified by reverse transcriptase-polymerase chain reaction analysis of brain mRNA extracts. The mRNA was also detected in cultured postmortem human microglia and in the IMR-32 human neuroblastoma cell line. Immunohistochemically,...
The effect of cleavage of Cl-inhibitor at Pro 36 by a Crotalus atrox α-proteinase fraction on the properties of this serpin was studied. This truncated Cl-inhibitor (des 1–36) was fully active as an inhibitor of kallikrein, β-factor XIIa, and Cl̄s, and modulated the functions of Cl in a normal manner. Also, the half-life of the truncated protein in the circulation of rabbits, both alone and...
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