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[Ca2+]i alterations are vital in signaling pathways of cell activation. We tried to detect such changes, in intracellular signaling pathways downstream TLR4 in immune cells, following stimulation with prothymosin alpha (proTα) and its decapeptide proTα(100–109). Human leukocytes were activated with LPS, proTα or proTα(100–109), directly or after 24h stimulation, while neutrophils were directly challenged...