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Confocal Laser Scanning Microscopy (CLSM) technology and bioimaging are powerful tools for three-dimensional (3D) and colocalization molecular analysis of the microspore embryogenesis. Strategies with fluorescent-labelled probes for in situ hybridization and immunofluorescence have provided unique images of the spatial and temporal pattern of the expression of genes and proteins, and of the sub-cellular...
Methylation-acetylation (MA) is an easy and reproducible ultrastructural cytochemical method which gives preferential contrast to nucleic acid containing structures. When performed en bloc before Lowicryl embedding it does not affect the main antigenic and chemical properties of the sample and is compatible with a large variety of modern immunogold methods permitting a better assignment of the labeling...
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