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The interleukin‐17 (IL‐17) cytokines, IL‐17A to IL‐17F, are emerging as critical players in host defence responses and inflammatory diseases. Substantial data support the role of these proteins in innate and adaptive immunity. Of these family members, IL‐17A, IL‐17F and IL‐17E have been the best studied. Both IL‐17A and IL‐17F contribute to the host response to extracellular bacteria and fungi, and...
Introduction Accumulating evidence suggests that the interleukin (IL)-17 cytokines are major players in the immune response to foreign pathogens. In addition, the pathogeneses of a number of inflammatory diseases have been linked to uncontrolled expression of these cytokine pathways. Discussion Genetic and biochemical analyses have elucidated the cellular and molecular events triggered by these...
A gel-based fluorescence resonance energy transfer (gelFRET) assay was developed for analysis of the architecture of nucleoprotein complexes. gelFRET is based on fluorescence analysis of nucleoprotein complexes separated by polyacrylamide gel electrophoresis. These complexes are separated from free components and nonspecific complexes, enabling fluorescence analysis of complexes containing all components...
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