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Toluene dioxygenase-catalysed (TDO) oxidation converts substituted phenol substrates into catechols, hydroquinones, and chiral cyclohexenone cis-diol products. The ratio between the isolated products varied widely even between similar substrates, e.g. o-cresol, m-cresol and p-cresol. These differences are caused by different binding interactions within the active site of TDO. This study provides insight...
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