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Gliadins are a large and complex group of proteins, consisting of dozens to hundreds of unique polypeptides in any wheat cultivar. Individual gliadins differ in molecular weight, isoelectric point, and amino acid sequences, making their analyses especially difficult. We here used preparative polyacrylamide gel electrophoresis in acidic conditions to fractionate gliadins of the cultivar Fraza. Resulting...
Gliadin proteins of two winter wheat genotypes (the cultivar Ostka strzelecka and the line 60A) were analysed by ELISA using serum with a high content of IgE antibodies to gluten, obtained from patients showing symptoms of food and skin allergies. These wheats differed strongly in gliadin composition and gluten rheological properties. Two extraction methods were employed: a single-step method using...
In persons showing gluten intolerance, wheat storage proteins (gliadins and glutenins) cause IgE-mediated allergies and coeliac disease. Because both of these protein fractions play a significant role in the human diet, intensive research has been carried out to explore the biochemical and molecular reasons for their allergenicity, and the possibilities for eliminating or limiting it. In spite of...
Wykonano analizy cytologiczne kariotypów karłowych mutantów pszenżyta ozimego oraz ich form wyjściowych (kontrola) metodą C - prążkową, a także analizy białek zapasowych (glutenin wysokocząsteczkowych) metodą rozdziału elektroforetycznego na żelu poliakrymidowym z dodatkiem soli sodowej siarczanu dodecylu (SDS PAGE). Analizy cytologiczne wykazały, że wszystkie badane materiały miały pełny zestaw chromosomów...
Flours of four common wheat and one spelt genotypes were treated with thioredoxin-h (TR-h) in the presence of NADPH and NADP-thiore- doxin reductase to reduce intramolecular disulphide bonds in monomeric sulphur-rich prolamins. Effects on the breadmaking properties (determined by micro-baking) and gliadin's immunoreactivity (measured by Sandwich ELISA) were observed. Loaf volumes and estimated crumb...
Z uwagi na niską zawartość pewnych składników biochemicznych ziarna wartość odżywcza pszenicy jest gorsza w porównaniu z innymi gatunkami roślin uprawnych. Przyjęto założenie, iż tę cechę można poprawić przenosząc geny kodujące białka gliadynowe z Tr. spelta do Tr. vulgare. Spośród mieszańców orkiszu i odmiany Elena wyprowadzono linie bliskoizogeniczne różniące się niektórymi podjednostkami należącymi...
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