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Haploidisation is a biotechnological method used to obtain plants with improved traits that are of use to humans. Lettuce (Lactuca sativa L.), a well-known and popular leafy vegetable, is consumed worldwide. Its haploid form would provide a good basis for producing a pure line of plants (doubled haploids) allowing new varieties to be regenerated. The main aim of this work was to develop an effective...
This paper is a short review of historical development of the tissue culture method in Poland. Similarly to the rest of the world, in vitro technology in Poland has progressed in many directions simultaneously. Its main fields are closely interconnected by natural sequences of biological processes and integrade one into another. The best results, driven by the prospects of practical applications,...
We used germination tests to assess the frequency of polyembryony in 9 asparagus cultivars with a high propensity to produce double embryos with different ploidy levels: Alpha, Andreas, Boonlim, Cipres, Eposs, Helios, Limbras, Ravel and Sartaguda. Twin embryos inside a single seed were found in 3 cultivars: Eposs 2n, Ravel 2n and Sartaguda 2n, at 0.60% frequency (15 seeds with twin embryos out of...
W pracy dokonano oceny potencjału regeneracyjnego pręcików i słupków kwiatu gorczycy białej (Sinapis alba L.) oraz sprawdzenie ich przydatności do mikrorozmnażania. Eksplantaty wykładano na pożywkę regeneracyjną MS z dodatkiem 3 mg·dm⁻³ BAP i 1,6 mg·dm⁻³ 2,4-D. Pędy przybyszowe ukorzeniano na pożywce MS z dodatkiem 1 mg·dm⁻³ IAA. Po 75 dniach otrzymane rośliny wysadzano do doniczek. Stwierdzono, że...
The factors regulating callus proliferation and bud regeneration from stigma tissues are not sufficiently understood. To study the regenerative capacity of pistil elements, pistil of broccoli was cultured under simple culture conditions. Stigmas with style from broccoli pistils undergo somatic embryogenesis on Murashige and Skoog basal medium. Callus initiation occurred on basal medium supplemented...
Placentae of Melandrium album excised from ovaries of unopened flowers were pollinated in vitro with pollen grains of Lychnis coronaria. Pollen grains germinated, and pollen tubes were found entering the micropyles 18 h after pollination. Double fertilization occurred. Immature and mature hybrid embryos at different stages of development were analyzed up to the third week of culture. Twenty-five days...
For the first time, hybrid embryos and plantlets were obtained from in vitro-pollinated whole catkins of Salix viminalis with pollen grains of Populus tremula, P. tomentosa and P. alba. Only hybrid embryos were obtained from cross S. viminalis with P. lasiocarpa. Pollen of poplar species germinated on stigmas of willow and some pollen tubes were observed growing into the ovary. After 36–48 h of pollination,...
2 mm explants of immature inflorescences (0.5-3) produced calluses on MS medium with o.5 mg x 1 ?1 BAP and 2.5 mg x 1-1 2.4-D. The surface sterilization in 10% sodium hypochloride for 20 min was successful in 74%. After 20 days of incubation, the surfaces of all explants were covered by callus. After 2 months of culture, from most of calluses embryo-like structures developed. The highest frequency...
Pollen grains of Pinus wallichiana, P. mugo, P. ponderosa and Ephedra distachya germinated at various intensities on in vitro cultured placentas of 32 angiosperm species. Pollen of the same gymnosperms did not form tubes on stigmas of pistils cultured under the same conditions as the placentas. Pollen of several angiosperms germinated on semi in vitro cultured opened ovules of Larix decidua and nucelli...
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