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The N-terminal cluster binding motif Cys 8 XXXXXXXCys 16 ....Cys 49 of Bacillus schlegelii 7Fe ferredoxin, which provides the ligands to the [Fe 3 S 4 ] + cluster, was modified by the mutation Asp 13 →Cys. The mutant D13C is expressed in Escherichia coli as an 8Fe ferredoxin, with NMR properties similar to those of clostridial-type ferredoxins...
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