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Ultrasensitive and reliable DNA detection tools are currently needed for the diagnostic of infectious and genetic diseases. To achieve the required sensitivity, one can amplify either the target DNA or the signal generated by each target molecule detected. Since enzymatic amplification of DNA is prone to contamination or inhibition, sensors that can benefit from signal amplification are usually more...
A fluorescence signal amplification mechanism allowing detection limits for DNA in the zeptomolar range was investigated. Photophysical properties of the molecular system were studied in order to better explain the signal amplification that is observed. We show that the confinement of a fluorescent DNA hybridization transducer in aggregates improves its quantum yield and photostability. Furthermore,...
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