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Coomassie brilliant blue G-250 (CB) is the dye used frequently in the Bradford assay for protein concentration determination. In this study, we investigated how the solvent polarity and viscosity affect the CB absorption and fluorescence spectra and apply this understanding to investigate the binding of CB to lysozyme and insulin in the native and amyloid fibril states. Coomassie blue binds both to...
The spectroscopic properties of 2-[4′-(dimethylamino)phenyl]-benzothiazole (BTA-2) in solution and in the presence of amyloid fibrils were investigated using absorption and fluorescence spectroscopy. Solution studies show that BTA-2 forms micelles in aqueous solutions, but that the dye can be solvated upon the addition of acetonitrile (CH3CN). BTA-2 binds to amyloid fibrils in solution leading to...
The thermodynamics of the alpha (α) phase to beta (β) phase transition was investigated in solution of poly(9,9′-dioctylfluorene) in a variety of solvents with UV–vis absorption spectroscopy, differential scanning calorimetry, fluorescence spectroscopy, atomic force microscopy, and near-field scanning optical microscopy. The results show that the solvent quality has a strong affect on the α- to β-conformational...
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