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In article number 2003912, Marcelina Cardoso Dos Santos, Niko Hildebrandt, and co‐workers develop terbium‐to‐quantum‐dot fluorescence resonance energy transfer (FRET) nanohybrids for bright and autofluorescence‐free multiplexed imaging in live cells and long‐term imaging in developing zebrafish embryos over seven days, upon one‐cell stage microinjection. The work provides a strategy for advancing...
The zebrafish is an important vertebrate model for disease, drug discovery, toxicity, embryogenesis, and neuroscience. In vivo fluorescence microscopy can reveal cellular and subcellular details down to the molecular level with fluorescent proteins (FPs) currently the main tool for zebrafish imaging. However, long maturation times, low brightness, photobleaching, broad emission spectra, and sample...
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