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A novel formulation of biodegradable microparticles was developed for the sustained release of peptide and protein drugs. The microparticles were formed by the aggregation of protein nanoparticles through water-in-oil (W/O) emulsion-lyophilization and subsequent solid-in-oil-in-water (S/O/W) emulsion–solvent evaporation. Amphiphilic copolymers were used as an emulsifier in the W/O emulsion and matrix...
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