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Several recent micro- and nanotechnologies have provided novel methods for biological studies of adherent cells because the small features of these new biotools provide unique capabilities for accessing cells without the need for suspension or lysis. These novel approaches have enabled gentle but effective delivery of molecules into specific adhered target cells, with unprecedented spatial resolution...
The transfection of molecules into cultured cells is a critical step toward fundamental studies of cells, drug screening, and stem cell reprogramming/differentiation. Despite rapid development of transfection methods, efficient transfection of adherent cells while maintaining viability is still challenging, in particular, for sensitive primary cells. To achieve this goal, we present a microfluidic...
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