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An approach to the modification and post‐fabrication chemical patterning of amine‐reactive superhydrophobic surfaces is reported. The approach is based on the layer‐by‐layer fabrication of azlactone‐containing polymer multilayers, and permits the direct covalent attachment and subsequent patterning of chemical and biological “inks” using a variety of pratical and low‐cost pattern‐transfer methods...
Our study demonstrates that substrates fabricated using a “reactive” layer‐by‐layer approach promote well‐defined cell–substrate interactions of human corneal epithelial cells. Specifically, crosslinked and amine‐reactive polymer multilayers were produced by alternating “reactive” deposition of an azlactone‐functionalized polymer [poly(2‐vinyl‐4,4‐dimethylazlactone)] (PVDMA) and a primary amine‐containing...
Fibrin-based sealants are commonly employed to arrest bleeding after surgery. Usually, fibrinogen obtained from pooled human plasma is used to prepare sealants, with attendant risk of blood-borne infections. Availability of autologous fibrinogen would eliminate this risk. To prepare autologous fibrin sealant, fibrinogen was precipitated from human plasma using protamine. Under optimal conditions (10-mg/mL...
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