Search results for: Udayaditya Sen

Items from 1 to 20 out of 22 results

article

Crystal structure of phosphoglucomutase from Leishmania major at 3.5 Å resolution

Barnali Waugh, Udayaditya Sen, Rahul Banerjee

Biochimie > 2016 > 121 > C > 102-111

The crystal structure of phosphoglucomutase (LmPGM) from the parasite Leishmania major has been solved at 3.5 Å resolution. Although the active form of the enzyme is monomeric in solution, four molecules (A, B, C, D) were found in the asymmetric unit, of which the pairs (A,D) and (B,C) were of identical inter-subunit geometry. The parasitic enzyme constituted of four domains exhibited the canonical...

article

A conserved tryptophan (W91) at the barrel-lid junction modulates the packing and stability of Kunitz (STI) family of inhibitors

Sudip Majumder, Susmita Khamrui, Ramanuj Banerjee, Pallab Bhowmik, more

BBA - Proteins and Proteomics > 2015 > 1854 > 1 > 55-64

β-trefoil fold, consisting of a six stranded β-barrel capped at one end by a lid comprising of another six β-strands, is one of the most important folds among proteins. Important classes of proteins like Interleukins (ILs), Fibroblast Growth Factors (FGFs), Kunitz (STI) family of inhibitors etc. belong to this fold. Their core is packed by hydrophobic residues contributed by the 6 stranded β-barrel...

article

Crystallization and preliminary X‐ray analysis of a ribokinase from Vibrio cholerae O395

Rakhi Paul, Madhumita Dandopath Patra, Ramanuj Banerjee, Udayaditya Sen

Acta Crystallographica Section F > 70 > 8 > 1098 - 1102

Ribokinase (RK) is one of the principal enzymes in carbohydrate metabolism, catalyzing the reaction of D‐ribose and adenosine triphosphate to produce ribose‐5‐phosphate and adenosine diphosphate (ADP). To provide further insight into the catalytic mechanism, the rbsK gene from Vibrio cholerae O395 encoding ribokinase was cloned and the protein was overexpressed in Escherichia coli BL21 (DE3) and purified...

article

Atomic resolution crystal structure of VcLMWPTP-1 from Vibrio cholerae O395: Insights into a novel mode of dimerization in the low molecular weight protein tyrosine phosphatase family

Seema Nath, Ramanuj Banerjee, Udayaditya Sen

Biochemical and Biophysical Research Communications > 2014 > 450 > 1 > 390-395

Low molecular weight protein tyrosine phosphatase (LMWPTP) is a group of phosphotyrosine phosphatase ubiquitously found in a wide range of organisms ranging from bacteria to mammals. Dimerization in the LMWPTP family has been reported earlier which follows a common mechanism involving active site residues leading to an enzymatically inactive species. Here we report a novel form of dimerization in...

article

A Novel 8-nm Protein Cage Formed by Vibrio cholerae Acylphosphatase

Seema Nath, Ramanuj Banerjee, Udayaditya Sen

Journal of Molecular Biology > 2014 > 426 > 1 > 36-38

Here we show the formation of an ~8-nm cage formed by the self-assembly of acylphosphatase from Vibrio cholerae O395 (Vc-AcP). The 12-subunit cage structure forms spontaneously and is stabilized through binding of sulfate ions at its exterior face and interfacial regions. Crystal structure and studies in solutions illuminate the basis for the formation of the cage, while a single (Cys20→Arg) mutation...

article

Cloning, expression, purification, crystallization and preliminary X‐ray analysis of a fructokinase from Vibrio cholerae O395

Rakhi Paul, Seema Nath, Udayaditya Sen

Acta Crystallographica Section F > 68 > 12 > 1564 - 1567

Fructokinase (FK), one of the crucial enzymes for sugar metabolism in bacterial systems, catalyses the unidirectional phosphorylation reaction from fructose to fructose 6‐phosphate, thereby allowing parallel entry of fructose into glycolysis beside glucose. The cscK gene from Vibrio cholerae O395 coding for the enzyme FK has been cloned, overexpressed in Escherichia coli BL21 (DE3) and purified using...

article

Cloning, purification, crystallization and preliminary X‐ray analysis of two low‐molecular‐weight protein tyrosine phosphatases from Vibrio cholerae

Seema Nath, Ramanuj Banerjee, Susmita Khamrui, Udayaditya Sen

Acta Crystallographica Section F > 68 > 10 > 1204 - 1208

Low‐molecular‐weight protein tyrosine phosphatases (LMWPTPs) are small cytoplasmic enzymes of molecular weight ∼18 kDa that belong to the large family of protein tyrosine phosphatases (PTPs). Despite their wide distribution in both prokaryotes and eukaryotes, their exact biological role in bacterial systems is not yet clear. Two low‐molecular‐weight protein tyrosine phosphatases (VcLMWPTP‐1 and Vc...

article

Biophysical studies with AICD-47 reveal unique binding behavior characteristic of an unfolded domain

Samir Das, Saptaparni Ghosh, Dipak Dasgupta, Udayaditya Sen, more

Biochemical and Biophysical Research Communications > 2012 > 425 > 2 > 201-206

APP intracellular C-terminal domain (AICD-47), generated upon γ-secretase cleavage of amyloid precursor’s protein (APP), bears the signature of a classical intrinsically unstructured domain (IUD). Comparing the recent crystal structures of AICD-47 peptides bound to its different adaptors such as protein-tyrosine-binding domain-2 (PTB2) of Fe65 and Src homology 2 (SH2) domain of growth factor receptor...

article

Role of remote scaffolding residues in the inhibitory loop pre-organization, flexibility, rigidification and enzyme inhibition of serine protease inhibitors

Sudip Majumder, Susmita Khamrui, Jhimli Dasgupta, Jiban K. Dattagupta, more

BBA - Proteins and Proteomics > 2012 > 1824 > 7 > 882-890

Canonical serine protease inhibitors interact with cognate enzymes through the P3–P2′ region of the inhibitory loop while its scaffold hardly makes any contact. Neighboring scaffolding residues like Arginines or Asparagine shape-up the inhibitory loop and favor the resynthesis of cleaved scissile bond. However, role of remote scaffolding residues, which are not involved in religation, was not properly...

article

Overexpression, purification, crystallization and preliminary X‐ray analysis of CheY4 from Vibrio cholerae O395

Maitree Biswas, Susmita Khamrui, Udayaditya Sen, Jhimli Dasgupta

Acta Crystallographica Section F > 67 > 12 > 1645 - 1648

Chemotaxis and motility greatly influence the infectivity of Vibrio cholerae, although the role of chemotaxis genes in V. cholerae pathogenesis is poorly understood. In contrast to the single copy of CheY found in Escherichia coli and Salmonella typhimurium, four CheYs (CheY1–CheY4) are present in V. cholerae. While insertional disruption of the cheY4 gene results in decreased motility, insertional...

article

Functional Implications of the Conformational Switch in AICD Peptide upon Binding to Grb2-SH2 Domain

Samir Das, Mithu Raychaudhuri, Udayaditya Sen, Debashis Mukhopadhyay

Journal of Molecular Biology > 2011 > 414 > 2 > 217-230

It has been hypothesized previously that synergistic effect of both amyloid precursor protein intracellular C-terminal domain (AICD) and Aβ aggregation could contribute to Alzheimer's disease pathogenesis. Structural studies of AICD have found no stable globular fold over a broad range of pH. Present work is based on the premises that a conformational switch involving the flipping of C-terminal helix...

article

Cloning, expression, purification, crystallization and preliminary X‐ray analysis of the 31 kDa Vibrio cholerae heat‐shock protein VcHsp31

Samir Das, Sanjay Dey, Trina Roy, Udayaditya Sen

Acta Crystallographica Section F > 67 > 11 > 1382 - 1385

The Gram‐negative bacterium Vibrio cholerae, which is responsible for the diarrhoeal disease cholera in humans, induces the expression of numerous heat‐shock genes. VcHsp31 is a 31 kDa putative heat‐shock protein that belongs to the DJ‐1/PfpI superfamily, functioning as both a chaperone and a protease. VcHsp31 has been cloned, overexpressed and purified by Ni²⁺–NTA affinity chromatography followed...

article

Cloning, overexpression, purification, crystallization and preliminary X‐ray analysis of CheY3, a response regulator that directly interacts with the flagellar `switch complex' in Vibrio cholerae

Susmita Khamrui, Maitree Biswas, Udayaditya Sen, Jhimli Dasgupta

Acta Crystallographica Section F > 66 > 8 > 944 - 947

Vibrio cholerae is the aetiological agent of the severe diarrhoeal disease cholera. This highly motile organism uses the processes of motility and chemotaxis to travel and colonize the intestinal epithelium. Chemotaxis in V. cholerae is far more complex than that in Escherichia coli or Salmonella typhimurium, with multiple paralogues of various chemotaxis genes. In contrast to the single copy of the...

article

Structures of Grb2-AICD peptides reveal a conformational switch

Samir Das, Udayaditya Sen, Debashis Mukhopadhyay

Alzheimer's & Dementia > 2010 > 6 > 4(S) > S381

article

Crystallization and preliminary X‐ray analysis of Psu, an inhibitor of the bacterial transcription terminator Rho

Susmita Khamrui, Amitabh Ranjan, Bibhusita Pani, Ranjan Sen, more

Acta Crystallographica Section F > 66 > 2 > 204 - 206

Psu, a coat protein from bacteriophage P4, inhibits Rho‐dependent transcription termination both in vivo and in vitro. The Psu protein is α‐helical in nature and appeared to be a dimer in solution. It interacts with Rho and affects the ATP binding and RNA‐dependent ATPase activity of Rho, which in turn reduces the rate of RNA release from the elongation complex. Crystals of Psu were grown in space...

article

Crystal Structure of the Human Lymphoid Tyrosine Phosphatase Catalytic Domain: Insights into Redox Regulation,

Sophia J. Tsai, Udayaditya Sen, Lei Zhao, William B. Greenleaf, more

Biochemistry > 2009 > 48 > 22 > 4838 - 4845

article

Spacer Asn Determines the Fate of Kunitz (STI) Inhibitors, as Revealed by Structural and Biochemical Studies on WCI Mutants ^‡

Jhimli Dasgupta, Susmita Khamrui, Jiban K. Dattagupta, Udayaditya Sen

Biochemistry > 2006 > 45 > 22 > 6783 - 6792

article

Single mutation at P1 of a chymotrypsin inhibitor changes it to a trypsin inhibitor: X-ray structural (2.15 Å) and biochemical basis

Susmita Khamrui, Jhimli Dasgupta, Jiban K. Dattagupta, Udayaditya Sen

BBA - Proteins and Proteomics > 2005 > 1752 > 1 > 65-72

Change in specificity, caused by the mutations at P1 site, of the serine protease inhibitors of different families is reported in the literature, but Kunitz (STI) family inhibitors are almost unexplored in this regard. In this paper, we present the crystal structure of a P1 variant of winged bean chymotrypsin inhibitor (WCI) belonging to Kunitz (STI) family, supplemented by biochemical, phylogenetic...

article

Crystal Structures of HbA ₂ and HbE and Modeling of Hemoglobin δ ₄ : Interpretation of the Thermal Stability and the Antisickling Effect of HbA ₂ and Identification of the Ferrocyanide Binding Site in Hb ^‡

Udayaditya Sen, Jhimli Dasgupta, Debi Choudhury, Poppy Datta, more

Biochemistry > 2004 > 43 > 39 > 12477 - 12488

article

Crystallization and preliminary X-ray structural studies of hemoglobin A ₂ and hemoglobin E, isolated from the blood samples of β-thalassemic patients

Jhimli Dasgupta, Udayaditya Sen, Debi Choudhury, Poppy Datta, more

Biochemical and Biophysical Research Communications > 2003 > 303 > 2 > 619-623

Hemoglobin A ₂ (α ₂ δ ₂ ), a minor (2-3%) component of circulating red blood cells, acts as an anti-sickling agent and its elevated concentration in β-thalassemia is a useful clinical diagnostic. In β-thalassemia major, where there is a failure of β-chain production, HbA ₂ acts as the predominant oxygen deliverer. Hemoglobin E, is another common abnormal hemoglobin,...

Publication date

Set your own date range

Keywords

VIBRIO CHOLERAE (4)
ATP (2)
BACTERIAL PROTEIN PHOSPHATASES (2)
CONFORMATIONAL SWITCH (2)
X-RAY CRYSTALLOGRAPHY (2)
HEMOGLOBIN A 2 (1)
ACTIVE DIMER (1)
AICD-47 (1)
ALZHEIMER'S DISEASE (1)
ALZHEIMER’S DISEASE (1)
CAVITY (1)
CHAPERONES (1)
CHEMOTAXIS (1)
CHIMERIC TRYPSIN INHIBITOR (1)
CHYMOTRYPSIN INHIBITOR (1)
COLONIZATION (1)
CRYSTALLIZATION (1)
CRYSTALLOGRAPHY (1)
DEPHOSPHORYLATION (1)
DOCKING (1)
DOCKING STUDY (1)
DRUG DESIGNING (1)
DYNAMIC LIGHT SCATTERING (1)
EΒ-THALASSEMIA (1)
FLAGELLAR ROTATION (1)
FLUORINATED ALCOHOL (1)
FRUCTOKINASE (1)
FRUCTOSE (1)
GRB2 (1)
HEAT‐SHOCK PROTEINS (1)
HEMOGLOBIN E (1)
HSP31 (1)
HYDROPHOBIC CORE (1)
INHIBITOR LOOP FLEXIBILITY (1)
INTRINSICALLY UNSTRUCTURED PROTEIN (1)
KUNITZ (STI) INHIBITORS (1)
LEISHMANIA MAJOR (1)
LOOP AND SCAFFOLD (1)
LOOP RIGIDIFICATION (1)
LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASES (1)
MOLECULAR RECOGNITION (1)
MOTILITY (1)
NORMAL MODE ANALYSIS (1)
PEPTIDASES (1)
PHOSPHOGLUCOMUTASE (1)
PHOSPHORYLATED TYROSINE (1)
PHOSPHORYLTRANSFER (1)
PHOSPHOTYROSINE (1)
PROTEIN CRYSTALLIZATION (1)
PROTEIN SELF-ASSEMBLY (1)
PROTEIN TYROSINE KINASES (1)
PROTEIN TYROSINE PHOSPHATASES (1)
PSU (1)
PURIFICATION (1)
RHO (1)
RIBOKINASE (1)
RIBOSE (1)
SH2 (1)
SITE DIRECTED MUTAGENESIS (1)
SPECIFICITY CHANGE (1)
TRANSCRIPTION TERMINATION (1)
TRYPTOPHAN (W91) (1)
X-RAY CRYSTALLOGRAPHIC STUDIES (1)
X-RAY STRUCTURE (1)
Α-PHOSPHOHEXOMUTASE SUPERFAMILY (1)
Β-THALASSEMIA (1)
Β-TREFOIL FOLD (1)
more

Data set

Elsevier (11)
Wiley (7)
ACS (4)

Journal

Acta Crystallographica Section F (7)
Biochemistry (4)
BBA - Proteins and Proteomics (3)
Biochemical and Biophysical Research Communications (3)
Journal of Molecular Biology (2)
Alzheimer's & Dementia (1)
Biochimie (1)
Structure (1)

INFONA - science communication portal

Search results for: Udayaditya Sen

Crystal structure of phosphoglucomutase from Leishmania major at 3.5 Å resolution

A conserved tryptophan (W91) at the barrel-lid junction modulates the packing and stability of Kunitz (STI) family of inhibitors

Crystallization and preliminary X‐ray analysis of a ribokinase from Vibrio cholerae O395

Atomic resolution crystal structure of VcLMWPTP-1 from Vibrio cholerae O395: Insights into a novel mode of dimerization in the low molecular weight protein tyrosine phosphatase family

A Novel 8-nm Protein Cage Formed by Vibrio cholerae Acylphosphatase

Cloning, expression, purification, crystallization and preliminary X‐ray analysis of a fructokinase from Vibrio cholerae O395

Cloning, purification, crystallization and preliminary X‐ray analysis of two low‐molecular‐weight protein tyrosine phosphatases from Vibrio cholerae

Biophysical studies with AICD-47 reveal unique binding behavior characteristic of an unfolded domain

Role of remote scaffolding residues in the inhibitory loop pre-organization, flexibility, rigidification and enzyme inhibition of serine protease inhibitors

Overexpression, purification, crystallization and preliminary X‐ray analysis of CheY4 from Vibrio cholerae O395

Functional Implications of the Conformational Switch in AICD Peptide upon Binding to Grb2-SH2 Domain

Cloning, expression, purification, crystallization and preliminary X‐ray analysis of the 31 kDa Vibrio cholerae heat‐shock protein VcHsp31

Cloning, overexpression, purification, crystallization and preliminary X‐ray analysis of CheY3, a response regulator that directly interacts with the flagellar `switch complex' in Vibrio cholerae

Structures of Grb2-AICD peptides reveal a conformational switch

Crystallization and preliminary X‐ray analysis of Psu, an inhibitor of the bacterial transcription terminator Rho

Crystal Structure of the Human Lymphoid Tyrosine Phosphatase Catalytic Domain: Insights into Redox Regulation,

Spacer Asn Determines the Fate of Kunitz (STI) Inhibitors, as Revealed by Structural and Biochemical Studies on WCI Mutants ^‡

Single mutation at P1 of a chymotrypsin inhibitor changes it to a trypsin inhibitor: X-ray structural (2.15 Å) and biochemical basis

Crystal Structures of HbA ₂ and HbE and Modeling of Hemoglobin δ ₄ : Interpretation of the Thermal Stability and the Antisickling Effect of HbA ₂ and Identification of the Ferrocyanide Binding Site in Hb ^‡

Crystallization and preliminary X-ray structural studies of hemoglobin A ₂ and hemoglobin E, isolated from the blood samples of β-thalassemic patients

Filter options

Publication date

Keywords

Data set

Journal

INFONA - science communication portal

Search results for: Udayaditya Sen

Add recipient

Sending message cancelled

Are you sure you want to cancel sending this message?

Send message

Filter options

Publication date

Date range setting

Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.

Keywords

Data set

Journal

Reporting an error / abuse

Sending the report failed

Accessibility options