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Structural roles of functional Ca2+ and Cl− ions in photosynthetic oxygen-evolving complexes (OEC) were studied using low- (640–350 cm−1) and mid- (1800–1200 cm−1) frequency S2/S1 Fourier transform infrared (FTIR) difference spectroscopy. Studies using highly active Photosystem (PS) II core particles from spinach enabled the detection of subtle spectral changes. Ca2+-depleted and Ca2+-reconstituted...
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