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To generate hemoglobin-free full-length haptoglobin the cDNA encoding rat haptoglobin αβ subunits was cloned into shuttle vector pVT-Bac-His and used to produce a recombinant baculovirus Autographa californica Nuclear Polyhedrosis Virus (AcNPV) as an expression vector, named HpAcNPV. Recombinant virus was used to infect Spodoptera frugiperda (Sf9) insect cells. The 50kDa protein expressed was mostly...
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