Search results for: Andrew T. Koppisch

Items from 1 to 18 out of 18 results

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Integration of choline geranate into electrospun protein scaffolds affords antimicrobial activity to biomaterials used for cutaneous wound healing

Tatum A. Bardsley, Charlotte L. Evans, Joshua R. Greene, Robert Audet, more

Journal of Biomedical Materials Research Part B: Applied Biomaterials > 109 > 9 > 1271 - 1282

Wound healing attempts to maintain homeostasis in the wound while minimizing the risk of infection to the tissue by foreign agents, such as opportunistic bacterial pathogens. Biofilms established by these pathogens are a common cause of chronic infections that slow the healing process. Preparation of skin wound healing devices comprised of electrospun proteins associated with skin have been shown...

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Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts

Clifford J. Unkefer, Richard T. Sayre, Jon K. Magnuson, Daniel B. Anderson, more

Algal Research > 2017 > 22 > C > 187-215

In 2010, when the National Alliance for Advanced Biofuels and Bioproducts (NAABB) consortium began, little was known about the molecular basis of algal biomass or oil production. Very few algal genome sequences were available and efforts to identify the best-producing wild species through bioprospecting approaches had largely stalled after the U.S. Department of Energy's Aquatic Species Program. This...

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Structural characterization of polysaccharides expressed by Burkholderia oklahomensis E0147

Joshua K. Stone, Christian Heiss, Zhirui Wang, Ian Black, more

Carbohydrate Research > 2014 > 386 > Complete > 68-72

Burkholderia oklahomensis E0147 is a US isolated bacterium believed to express a similar O-antigen to type A structure of the highly pathogenic species, Burkholderia pseudomallei. Both species are genetically closely related. Lipopolysaccharide was collected from E0147 and structurally characterized to test this hypothesis. Glycosyl composition and linkage analyses in conjunction with 1D and 2D ...

article

Evaluation of ionic liquids on phototrophic microbes and their use in biofuel extraction and isolation

Katherine S. Lovejoy, Lauren E. Davis, Lisa M. McClellan, Antonietta M. Lillo, more

Journal of Applied Phycology > 2013 > 25 > 4 > 973-981

Multiple ionic liquids (ILs) were assessed for their ability to extract branched, unsaturated hydrocarbons from an aqueous medium. In addition, IL cytotoxicity studies were performed on two phototrophic microbes, Synechocystis sp. PCC6803 and Botryococcus braunii var Showa. The optimum IL for use in an isoprenoid hydrocarbon extraction may vary based on the biological source of the isoprenoids. Our...

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Tetraalkylphosphonium-Based Ionic Liquids for a Single-Step Dye Extraction/MALDI MS Analysis Platform

Katherine S. Lovejoy, Geraldine M. Purdy, Srinivas Iyer, Timothy C. Sanchez, more

Analytical Chemistry > 2011 > 83 > 8 > 2921 - 2930

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Limited thermal stability of imidazolium and pyrrolidinium ionic liquids

Rico E. Del Sesto, T. Mark McCleskey, Clay Macomber, Kevin C. Ott, more

Thermochimica Acta > 2009 > 491 > 1-2 > 118-120

Ionic liquids, with their vast applications, have been touted as being thermally stable to very high temperatures. However, decomposition not detected by standard TGA and NMR techniques are observed with spectroscopic techniques sensitive enough to see small amounts of impurities. Decomposition temperatures of common ionic liquids appear to occur at hundreds of degrees below those temperatures previously...

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The Missing Link in Petrobactin Biosynthesis: asbF Encodes a (−)-3-Dehydroshikimate Dehydratase ^†

David T. Fox, Kinya Hotta, Chu-Young Kim, Andrew T. Koppisch

Biochemistry > 2008 > 47 > 47 > 12251 - 12253

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Biosynthesis of the 3,4-Dihydroxybenzoate Moieties of Petrobactin by Bacillus anthracis

Andrew T. Koppisch, Kinya Hotta, David T. Fox, Christy E. Ruggiero, more

The Journal of Organic Chemistry > 2008 > 73 > 15 > 5759 - 5765

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Petrobactin is produced by both pathogenic and non-pathogenic isolates of the Bacillus cereus group of bacteria

Andrew T. Koppisch, Suraj Dhungana, Karen K. Hill, Hakim Boukhalfa, more

BioMetals > 2008 > 21 > 5 > 581-589

Petrobactin is the primary siderophore synthesized by Bacillus anthracis str Sterne and is required for virulence of this organism in a mouse model. The siderophore’s biosynthetic machinery was recently defined and gene homologues of this operon exist in several other Bacillus strains known to be mammalian pathogens, but are absent in several known to be harmless such as B. subtilis and B. lichenformis...

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Purification and characterization of rhodobactin: a mixed ligand siderophore from Rhodococcus rhodochrous strain OFS

Suraj Dhungana, Ryszard Michalczyk, Hakim Boukhalfa, Joseph G. Lack, more

BioMetals > 2007 > 20 > 6 > 853-867

The siderophore produced by Rhodococcus rhodochrous strain OFS, rhodobactin, was isolated from iron-deficient cultures and purified by a combination of XAD-7 absorptive/partition resin column and semi-preparative HPLC. The siderophore structure was characterized using 1D and 2D ¹H, ¹³C and ¹⁵N NMR techniques (DQFCOSY, TOCSY, NOESY, HSQC and LR-HSQC) and was confirmed using ESI-MS and MS/MS experiments...

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Proteomic analysis of Bacillus anthracis Sterne vegetative cells

Anthony W. Francis, Christy E. Ruggiero, Andrew T. Koppisch, Jingao Dong, more

BBA - Proteins and Proteomics > 2005 > 1748 > 2 > 191-200

Mass spectrometry and proteomics have found increasing use as tools for the rapid detection of pathogenic bacteria, even when they are in a mixture of non-pathogenic relatives. The success of this technique is greatly augmented by the availability of publicly accessible proteomic databases for specific pathogenic bacteria. To aid proteomic detection analyses for the causative agent of anthrax, we...

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Petrobactin is the Primary Siderophore Synthesized by Bacillus anthracis Str. Sterne under Conditions of Iron Starvation

Andrew T. Koppisch, Cindy C. Browder, Amanda L. Moe, Jacob T. Shelley, more

BioMetals > 2005 > 18 > 6 > 577-585

The siderophores of Bacillus anthracis are critical for the pathogen’s proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of ¹H and ¹³C NMR spectroscopy, UV spectroscopy...

article

The Acyltransferase Homologue from the Initiation Module of the R1128 Polyketide Synthase Is an Acyl-ACP Thioesterase That Edits Acetyl Primer Units ^†

Yi Tang, Andrew T. Koppisch, Chaitan Khosla

Biochemistry > 2004 > 43 > 29 > 9546 - 9555

article

Enhancing the Modularity of the Modular Polyketide Synthases: Transacylation in Modular Polyketide Synthases Catalyzed by Malonyl-CoA:ACP Transacylase

Pawan Kumar, Andrew T. Koppisch, David E. Cane, Chaitan Khosla

Journal of the American Chemical Society > 2003 > 125 > 47 > 14307 - 14312

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Structure-Based Mutagenesis of the Malonyl-CoA:Acyl Carrier Protein Transacylase from Streptomyces coelicolor ^†

Andrew T. Koppisch, Chaitan Khosla

Biochemistry > 2003 > 42 > 37 > 11057 - 11064

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Synthesis of 4-Diphosphocytidyl-2- C -methyl- d -erythritol and 2- C -Methyl- d -erythritol-4-phosphate

Andrew T. Koppisch, C. Dale Poulter

The Journal of Organic Chemistry > 2002 > 67 > 15 > 5416 - 5418

article

E. coli MEP Synthase: Steady-State Kinetic Analysis and Substrate Binding ^†

Andrew T. Koppisch, David T. Fox, Brian S. J. Blagg, C. D. Poulter

Biochemistry > 2002 > 41 > 1 > 236 - 243

article

The effects of secondary structure and O ₂ on the formation of direct strand breaks upon UV irradiation of 5-bromodeoxy-uridine-containing oligonucleotides

Gary P Cook, Tongqian Chen, Andrew T Koppisch, Marc M Greenberg

Chemistry & Biology > 1999 > 6 > 7 > 451-459

Background: 5-Bromodeoxyuridine is a radiosensitizing agent that is currently being evaluated in clinical trials as an adjuvant in the treatment of a variety of cancers. γ-Radiolysis and UV irradiation of oligonucleotides containing 5-bromodeoxyuridine result in the formation of direct strand breaks at the t'-adjacent nucleotide by oxidation of the respective deoxyribose. We investigated the effects...

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