Doreen E. Culham

article

Cardiolipin synthase A colocalizes with cardiolipin and osmosensing transporter ProP at the poles of Escherichia coli cells

Tatyana Romantsov, Karen Gonzalez, Naheda Sahtout, Doreen E. Culham, more

Molecular Microbiology > 107 > 5 > 623 - 638

Osmosensing by transporter ProP is modulated by its cardiolipin (CL)‐dependent concentration at the poles of Escherichia coli cells. Other contributors to this phenomenon were sought with the BACterial Two‐Hybrid System (BACTH). The BACTH‐tagged variants T18‐ProP and T25‐ProP retained ProP function and localization. Their interaction confirmed the ProP homo‐dimerization previously established by protein...

article

ProP‐ProP and ProP‐phospholipid interactions determine the subcellular distribution of osmosensing transporter ProP in Escherichia coli

Tatyana Romantsov, Doreen E. Culham, Tavia Caplan, Jennifer Garner, more

Molecular Microbiology > 103 > 3 > 469 - 482

Osmosensing transporter ProP protects bacteria from osmotically induced dehydration by mediating the uptake of zwitterionic osmolytes. ProP activity is a sigmoidal function of the osmolality. ProP orthologues share an extended, cytoplasmic C‐terminal domain. Orthologues with and without a C‐terminal, α‐helical coiled‐coil domain respond similarly to the osmolality. ProP concentrates at the poles and...

article

ProQ Is an RNA Chaperone that Controls ProP Levels in Escherichia coli

Steven G. Chaulk, Michelle N. Smith−Frieday, David C. Arthur, Doreen E. Culham, more

Biochemistry > 2011 > 50 > 15 > 3095 - 3106

article

Transmembrane Helix I and Periplasmic Loop 1 of Escherichia coli ProP Are Involved in Osmosensing and Osmoprotectant Transport

Robert A. B. Keates, Doreen E. Culham, Yaroslava I. Vernikovska, Adam J. Zuiani, more

Biochemistry > 2010 > 49 > 41 > 8847 - 8856

article

Roles of K ⁺ , H ⁺ , H ₂ O, and ΔΨ in Solute Transport Mediated by Major Facilitator Superfamily Members ProP and LacY ^†

Doreen E. Culham, Tatyana Romantsov, Janet M. Wood

Biochemistry > 2008 > 47 > 31 > 8176 - 8185

article

Structure and Function of Transmembrane Segment XII in Osmosensor and Osmoprotectant Transporter ProP of Escherichia coli ^†

Feng Liu, Doreen E. Culham, Yaroslava I. Vernikovska, Robert A. B. Keates, more

Biochemistry > 2007 > 46 > 19 > 5647 - 5655

article

Formation of an Antiparallel, Intermolecular Coiled Coil Is Associated with in Vivo Dimerization of Osmosensor and Osmoprotectant Transporter ProP in Escherichia coli

Alexander Hillar, Doreen E. Culham, Yaroslava I. Vernikovska, Janet M. Wood, more

Biochemistry > 2005 > 44 > 47 > 15636 - 15636

article

Formation of an Antiparallel, Intermolecular Coiled Coil Is Associated with in Vivo Dimerization of Osmosensor and Osmoprotectant Transporter ProP in Escherichia coli ^†

Alexander Hillar, Doreen E. Culham, Yaroslava I. Vernikovska, Janet M. Wood, more

Biochemistry > 2005 > 44 > 30 > 10170 - 10180

article

A Structural Model for the Osmosensor, Transporter, and Osmoregulator ProP of Escherichia coli ^†

Janet M. Wood, Doreen E. Culham, Alexander Hillar, Yaroslava I. Vernikovska, more

Biochemistry > 2005 > 44 > 15 > 5634 - 5646

article

Creation of a Fully Functional Cysteine-Less Variant of Osmosensor and Proton-Osmoprotectant Symporter ProP from Escherichia coli and Its Application to Assess the Transporter's Membrane Orientation ^†

Doreen E. Culham, Alexander Hillar, James Henderson, Anh Ly, more

Biochemistry > 2003 > 42 > 40 > 11815 - 11823

article

Osmosensor ProP of Escherichia coli Responds to the Concentration, Chemistry, and Molecular Size of Osmolytes in the Proteoliposome Lumen ^† ^, ^‡

Doreen E. Culham, James Henderson, Rebecca A. Crane, Janet M. Wood

Biochemistry > 2003 > 42 > 2 > 410 - 420

article

Requirements for Osmosensing and Osmotic Activation of Transporter ProP from Escherichia coli ^†

Kathleen I. Racher, Doreen E. Culham, Janet M. Wood

Biochemistry > 2001 > 40 > 24 > 7324 - 7333

article

The ion coupling and organic substrate specificities of osmoregulatory transporter ProP in Escherichia coli

Susan V. MacMillan, David A. Alexander, Doreen E. Culham, H. Jörg Kunte, more

BBA - Biomembranes > 1999 > 1420 > 1-2 > 30-44

Transporter ProP of Escherichia coli, a member of the major facilitator superfamily, mediates osmoprotective proline or glycine betaine accumulation by bacteria exposed to high osmolality environments. Morpholinopropane sulfonic acid, a common constituent of microbiological media, accumulates in osmoadapting E. coli cells but it is not osmoprotective and it did not influence proP transcription or...

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Search results for: Doreen E. Culham

Cardiolipin synthase A colocalizes with cardiolipin and osmosensing transporter ProP at the poles of Escherichia coli cells

ProP‐ProP and ProP‐phospholipid interactions determine the subcellular distribution of osmosensing transporter ProP in Escherichia coli

ProQ Is an RNA Chaperone that Controls ProP Levels in Escherichia coli

Transmembrane Helix I and Periplasmic Loop 1 of Escherichia coli ProP Are Involved in Osmosensing and Osmoprotectant Transport

Roles of K ⁺ , H ⁺ , H ₂ O, and ΔΨ in Solute Transport Mediated by Major Facilitator Superfamily Members ProP and LacY ^†

Structure and Function of Transmembrane Segment XII in Osmosensor and Osmoprotectant Transporter ProP of Escherichia coli ^†

Formation of an Antiparallel, Intermolecular Coiled Coil Is Associated with in Vivo Dimerization of Osmosensor and Osmoprotectant Transporter ProP in Escherichia coli

Formation of an Antiparallel, Intermolecular Coiled Coil Is Associated with in Vivo Dimerization of Osmosensor and Osmoprotectant Transporter ProP in Escherichia coli ^†

A Structural Model for the Osmosensor, Transporter, and Osmoregulator ProP of Escherichia coli ^†

Creation of a Fully Functional Cysteine-Less Variant of Osmosensor and Proton-Osmoprotectant Symporter ProP from Escherichia coli and Its Application to Assess the Transporter's Membrane Orientation ^†

Osmosensor ProP of Escherichia coli Responds to the Concentration, Chemistry, and Molecular Size of Osmolytes in the Proteoliposome Lumen ^† ^, ^‡

Requirements for Osmosensing and Osmotic Activation of Transporter ProP from Escherichia coli ^†

The ion coupling and organic substrate specificities of osmoregulatory transporter ProP in Escherichia coli

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