Search results for: Jade K. Forwood

Items from 1 to 20 out of 35 results

article

Structural determinants of phosphorylation‐dependent nuclear transport of HCMV DNA polymerase processivity factor UL44

Emily M. Cross, Oriano Marin, Daryl Ariawan, David Aragão, more

Human cytomegalovirus DNA polymerase processivity factor UL44 is transported into the nucleus by importin (IMP) α/β through a classical nuclear localization signal (NLS), and this region is susceptible to cdc2‐mediated phosphorylation at position T427. Whilst phosphorylation within and close to the UL44 NLS regulates nuclear transport, the details remain elusive, due to the paucity of structural information...

article

A functional and structural comparative analysis of large tumor antigens reveals evolution of different importin α‐dependent nuclear localization signals

Emily M. Cross, Nasim Akbari, Hanieh Ghassabian, Mikayla Hoad, more

Protein Science > 33 > 2 > n/a - n/a

Nucleocytoplasmic transport regulates the passage of proteins between the nucleus and cytoplasm. In the best characterized pathway, importin (IMP) α bridges cargoes bearing basic, classical nuclear localization signals (cNLSs) to IMPβ1, which mediates transport through the nuclear pore complex. IMPα recognizes three types of cNLSs via two binding sites: the major binding site accommodates monopartite...

article

Amyloid fibril formation, structure and domain swapping of acyl‐coenzyme A thioesterase‐7

Manjeet Kumar, Sarah L. Teakel, Crystall Swarbrick, Intifar S. Chowdhury, more

The FEBS Journal > 290 > 16 > 4057 - 4073

Acyl‐coenzyme A thioesterase (Acot) enzymes are involved in a broad range of essential intracellular roles including cell signalling, lipid metabolism, inflammation and the opening of ion channels. Dysregulation in lipid metabolism has been linked to neuroinflammatory and neurological disorders such as Alzheimer's and Parkinson's diseases. Structurally, Acot enzymes adopt a circularised trimeric arrangement...

article

Structural characterisation of a MAPR‐related archaeal cytochrome b_5M protein

Sarah Teakel, Michealla Marama, David Aragão, Sofiya Tsimbalyuk, more

FEBS Letters > 596 > 18 > 2409 - 2417

We recently reported that the membrane‐associated progesterone receptor (MAPR) protein family (mammalian members: PGRMC1, PGRMC2, NEUFC and NENF) originated from a new class of prokaryotic cytochrome b₅ (cytb₅) domain proteins, called cytb_5M (MAPR‐like). Relative to classical cytb₅ proteins, MAPR and ctyb_5M proteins shared unique sequence elements and a distinct heme‐binding orientation at an approximately...

article

Structural characterization of the porcine adeno‐associated virus Po1 capsid protein binding to the nuclear trafficking protein importin alpha

Mikayla Hoad, Justin A. Roby, Jade K. Forwood

FEBS Letters > 595 > 22 > 2793 - 2804

Adeno‐associated viruses (AAVs) are key vectors for gene therapy; thus, many aspects of their cell transduction pathway have been revealed in detail. However, the specific mechanisms AAV virions use to enter the host nucleus remain largely unresolved. We therefore aimed to reveal the structural interactions between the AAV capsid (Cap) protein and the nuclear transport protein importin alpha (IMPα)...

article

Structural characterization of β‐ketoacyl ACP synthase I bound to platencin and fragment screening molecules at two substrate binding sites

Edward I. Patterson, Jeffrey D. Nanson, Jan Abendroth, Cassie Bryan, more

Proteins: Structure, Function, and Bioinformatics > 88 > 1 > 47 - 56

The bacterial fatty acid pathway is essential for membrane synthesis and a range of other metabolic and cellular functions. The β‐ketoacyl‐ACP synthases carry out the initial elongation reaction of this pathway, utilizing acetyl‐CoA as a primer to elongate malonyl‐ACP by two carbons, and subsequent elongation of the fatty acyl‐ACP substrate by two carbons. Here we describe the structures of the β‐ketoacyl‐ACP...

article

Assessing circovirus gene flow in multiple spill-over events

Shubhagata Das, Kate Smith, Subir Sarker, Andrew Peters, more

Virus Genes > 2019 > 55 > 6 > 802-814

The establishment of viral pathogens in new host environments following spillover events probably requires adaptive changes within both the new host and pathogen. After many generations, signals for ancient cross-species transmission may become lost and a strictly host-adapted phylogeny may mimic true co-divergence while the virus may retain an inherent ability to jump host species. The mechanistic...

article

Nuclear import inhibitor N-(4-hydroxyphenyl) retinamide targets Zika virus (ZIKV) nonstructural protein 5 to inhibit ZIKV infection

Chunxiao Wang, Sundy N.Y. Yang, Kate Smith, Jade K. Forwood, more

Biochemical and Biophysical Research Communications > 2017 > 493 > 4 > 1555-1559

In the absence of approved therapeutics, Zika virus (ZIKV)'s recent prolific outbreaks in the Americas, together with impacts on unborn fetuses of infected mothers, make it a pressing human health concern worldwide. Although a key player in viral replication in the infected host cell cytoplasm, ZIKV non-structural protein 5 (NS5) appears to contribute integrally to pathogenesis by localising in the...

article

A novel pathogenic aviadenovirus from red-bellied parrots (Poicephalus rufiventris) unveils deep recombination events among avian host lineages

Shubhagata Das, Kathleen Fearnside, Subir Sarker, Jade K. Forwood, more

Virology > 2017 > 502 > C > 188-197

Competing roles of coevolution, selective pressure and recombination are an emerging interest in virus evolution. We report a novel aviadenovirus from captive red-bellied parrots (Poicephalus rufiventris) that uncovers evidence of deep recombination among aviadenoviruses. The sequence identity of the virus was most closely related to Turkey adenovirus D (42% similarity) and other adenoviruses in chickens,...

article

A comparison of PCR assays for beak and feather disease virus and high resolution melt (HRM) curve analysis of replicase associated protein and capsid genes

Shubhagata Das, Subir Sarker, Seyed Ali Ghorashi, Jade K. Forwood, more

Journal of Virological Methods > 2016 > 237 > C > 47-57

Beak and feather disease virus (BFDV) threatens a wide range of endangered psittacine birds worldwide. In this study, we assessed a novel PCR assay and genetic screening method using high-resolution melt (HRM) curve analysis for BFDV targeting the capsid (Cap) gene (HRM-Cap) alongside conventional PCR detection as well as a PCR method that targets a much smaller fragment of the virus genome in the...

article

Evolution of circoviruses in lorikeets lags behind its hosts

Shubhagata Das, Subir Sarker, Andrew Peters, Seyed A. Ghorashi, more

Molecular Phylogenetics and Evolution > 2016 > 100 > C > 281-291

The presence of endogenous viral elements in host genomes hints towards much older host–virus relationships than predicted by exogenous phylogenies, with highly mutable single-stranded DNA (ssDNA) viruses and RNA viruses often occupying entangled multispecies ecological niches. The difficulty lies in unravelling the long-term evolutionary history of vertebrate virus–host relationships and determining...

article

Structural Biology and Regulation of Protein Import into the Nucleus

Mary Christie, Chiung-Wen Chang, Gergely Róna, Kate M. Smith, more

Journal of Molecular Biology > 2016 > 428 > 10(PA) > 2060-2090

Proteins are translated in the cytoplasm, but many need to access the nucleus to perform their functions. Understanding how these nuclear proteins are transported through the nuclear envelope and how the import processes are regulated is therefore an important aspect of understanding cell function. Structural biology has played a key role in understanding the molecular events during the transport...

chapter

Nuclear Protein Import

David A. Jans, Jade K. Forwood

Molecular Biology Intelligence Unit > Nuclear Import and Export in Plants and Animals > 137-160

Entry into the eukaryotic cell nucleus occurs through multiple pathways involving specific targeting signals, and intracellular receptor molecules of the importin/karyopherin superfamily which recognise and dock the nuclear import substrates carrying these signals at the nuclear pore. Subsequent to translocation through the pore via a series of importin-mediated docking steps at multiple sites within...

article

Characterization of a beta-catenin nuclear localization defect in MCF-7 breast cancer cells

Cara Jamieson, Kate M. Mills, Christina Lui, Crystal Semaan, more

Experimental Cell Research > 2016 > 341 > 2 > 196-206

Beta-catenin plays a key role in transducing Wnt signals from the plasma membrane to the nucleus. Here we characterize an unusual subcellular distribution of beta-catenin in MCF-7 breast cancer cells, wherein beta-catenin localizes to the cytoplasm and membrane but atypically did not relocate to the nucleus after Wnt treatment. The inability of Wnt or the Wnt agonist LiCl to induce nuclear localization...

article

An efficient approach for recombinant expression and purification of the viral capsid protein from beak and feather disease virus (BFDV) in Escherichia coli

Subir Sarker, Seyed A. Ghorashi, Crystall M.D. Swarbrick, Yogesh B. Khandokar, more

Journal of Virological Methods > 2015 > 215-216 > Complete > 1-8

Structural insights into the biology of viruses such as beak and feather disease virus (BFDV) which do not replicate in cell cultures are increasingly reliant on recombinant methods for protein production and purification. Development of efficient methods for homogenous production of BFDV capsid protein is also essential for vaccine development and diagnostic purposes. In this study, two different...

article

Rapid genotyping of beak and feather disease virus using high-resolution DNA melt curve analysis

Subir Sarker, Seyed A. Ghorashi, Jade K. Forwood, Shane R. Raidal

Journal of Virological Methods > 2014 > 208 > Complete > 47-55

Beak and feather disease virus (BFDV) is a significant pathogen both for wild and captive psittacine birds globally. Genotypic differentiation of BFDV isolates is crucial to establish effective control strategies for the conservation of endangered species and epidemiological investigations of disease outbreaks. The technique developed in this study is a simple, rapid and inexpensive genotyping method...

article

Phylogeny of beak and feather disease virus in cockatoos demonstrates host generalism and multiple-variant infections within Psittaciformes

Subir Sarker, Seyed A. Ghorashi, Jade K. Forwood, Stephen J. Bent, more

Virology > 2014 > 460-461 > Complete > 72-82

Phylogenetic analyses of the highly genetically diverse but antigenically conserved, single-stranded circular, DNA genome of the avian circovirus, beak and feather disease virus (BFDV) from cockatoo species throughout Australia demonstrated a high mutation rate for BFDV (orders of magnitude fall in the range of 10 ^–4 substitutions/site/year) along with strong support for recombination indicating...

article

Functional disruption of yeast metacaspase, Mca1, leads to miltefosine resistance and inability to mediate miltefosine-induced apoptotic effects

Chayanika Biswas, Xiaoming Zuo, Sharon C.-A. Chen, Stephen D. Schibeci, more

Fungal Genetics and Biology > 2014 > 67 > Complete > 71-81

Miltefosine (MI) is a novel, potential antifungal agent with activity against some yeast and filamentous fungal pathogens. We previously demonstrated in the model yeast, Saccharomyces cerevisiae, that MI causes disruption of mitochondrial membrane potential and apoptosis-like cell death via interaction with the Cox9p sub-unit of cytochrome c oxidase (COX). To identify additional mechanisms of antifungal...

article

Purification, crystallization and preliminary X‐ray diffraction analysis of the N‐acetyltransferase SAV0826 from Staphylococcus aureus

Parul Srivastava, Yogesh B. Khandokar, Jade K. Forwood

Acta Crystallographica Section F > 70 > 2 > 211 - 214

Staphylococcus aureus is a prevalent microorganism that is capable of causing a wide range of infections and diseases. Several strains of this bacterial species have developed antibiotic resistance to methicillin and vancomycin, and higher death rates are still being reported each year owing to multidrug‐resistant strains. Certain GCN5‐related N‐acetyltransferases (GNATs) exhibit a broad substrate...

article

Expression, purification, crystallization and preliminary X‐ray analysis of the PaaI‐like thioesterase SAV0944 from Staphylococcus aureus

Yogesh B. Khandokar, Noelia Roman, Kate M. Smith, Parul Srivastava, more

Acta Crystallographica Section F > 70 > 2 > 244 - 247

Staphylococcus aureus is the causative agent of many diseases, including meningitis, bacteraemia, pneumonia, food poisoning and toxic shock syndrome. Structural characterization of the PaaI‐like thioesterase SAV0944 (SaPaaI) from S. aureus subsp. aureus Mu50 will aid in understanding its potential as a new therapeutic target by knowledge of its molecular details and cellular functions. Here, the recombinant...

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INFONA - science communication portal

Search results for: Jade K. Forwood

Structural determinants of phosphorylation‐dependent nuclear transport of HCMV DNA polymerase processivity factor UL44

A functional and structural comparative analysis of large tumor antigens reveals evolution of different importin α‐dependent nuclear localization signals

Amyloid fibril formation, structure and domain swapping of acyl‐coenzyme A thioesterase‐7

Structural characterisation of a MAPR‐related archaeal cytochrome b_5M protein

Structural characterization of the porcine adeno‐associated virus Po1 capsid protein binding to the nuclear trafficking protein importin alpha

Structural characterization of β‐ketoacyl ACP synthase I bound to platencin and fragment screening molecules at two substrate binding sites

Assessing circovirus gene flow in multiple spill-over events

Nuclear import inhibitor N-(4-hydroxyphenyl) retinamide targets Zika virus (ZIKV) nonstructural protein 5 to inhibit ZIKV infection

A novel pathogenic aviadenovirus from red-bellied parrots (Poicephalus rufiventris) unveils deep recombination events among avian host lineages

A comparison of PCR assays for beak and feather disease virus and high resolution melt (HRM) curve analysis of replicase associated protein and capsid genes

Evolution of circoviruses in lorikeets lags behind its hosts

Structural Biology and Regulation of Protein Import into the Nucleus

Nuclear Protein Import

Characterization of a beta-catenin nuclear localization defect in MCF-7 breast cancer cells

An efficient approach for recombinant expression and purification of the viral capsid protein from beak and feather disease virus (BFDV) in Escherichia coli

Rapid genotyping of beak and feather disease virus using high-resolution DNA melt curve analysis

Phylogeny of beak and feather disease virus in cockatoos demonstrates host generalism and multiple-variant infections within Psittaciformes

Functional disruption of yeast metacaspase, Mca1, leads to miltefosine resistance and inability to mediate miltefosine-induced apoptotic effects

Purification, crystallization and preliminary X‐ray diffraction analysis of the N‐acetyltransferase SAV0826 from Staphylococcus aureus

Expression, purification, crystallization and preliminary X‐ray analysis of the PaaI‐like thioesterase SAV0944 from Staphylococcus aureus

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