Search results for: Keng Zhu

Items from 1 to 9 out of 9 results

article

Thermal analysis reveals differential effects of various crosslinkers on bovine annulus fibrosis

Keng Zhu, Paul Slusarewicz, Tom Hedman

Journal of Orthopaedic Research® > 29 > 1 > 8 - 13

Treatment of a pathological spinal disc in vivo by injection of protein crosslinking reagents to restore the disc's mechanical properties is a new approach to the treatment of degenerative disc disease. In this study, the thermal stability of the collagen in disc annulus was measured by differential scanning calorimetry following treatment with six different crosslinking agents. The crosslinkers used...

article

Genipin-Based Tissue Modification for Degenerative Disc Disease

Tom Hedman, Bryan Kirking, Keng Zhu, Justin Toungate, more

The Spine Journal > 2010 > 10 > 9 Supplement > S40

article

Kinetic characterization and comparison of various protein crosslinking reagents for matrix modification

Paul Slusarewicz, Keng Zhu, Tom Hedman

Journal of Materials Science: Materials in Medicine > 2010 > 21 > 4 > 1175-1181

We have characterized the relative efficacies of a number of protein crosslinking agents that have the potential for use in the crosslinking of proteinaceous matrices both in vitro and in vivo. The crosslinkers tested were; l-threose (LT), Genipin (GP), Methylglyoxal (MG), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), proanthrocyanidin (PA) and glutaraldehyde (GA). The relative...

article

Large Disk Intermediate Precedes Formation of Apolipoprotein A-I−Dimyristoylphosphatidylcholine Small Disks ^†

Keng Zhu, Gregory Brubaker, Jonathan D. Smith

Biochemistry > 2007 > 46 > 21 > 6299 - 6307

article

Fluorescence spectroscopic characterization of Humicola lanuginosa lipase dissolved in its substrate

Arimatti Jutila, Keng Zhu, Esa K.J. Tuominen, Paavo K.J. Kinnunen

BBA - Proteins and Proteomics > 2004 > 1702 > 2 > 181-189

The conformational dynamics of Humicola lanuginosa lipases (HLL) and its three mutants were investigated by steady state and time-resolved fluorescence spectroscopy in two different media, aqueous buffer and the substrate triacetin. The fluorescence of the four Trps of the wild-type HLL (wt) reports on the global changes of the whole lipase molecule. In order to monitor conformational changes specifically...

article

Effects of betaine on the structural dynamics of Thermomyces (Humicola) lanuginosa lipase

Tim Söderlund, Keng Zhu, Arimatti Jutila, Paavo K.J. Kinnunen

Colloids and Surfaces B: Biointerfaces > 2002 > 26 > 1-2 > 75 - 83

The impact of betaine on the structural dynamics of a soluble protein, Thermomyces (Humicola) lanuginosa lipase (HLL) was studied using differential scanning calorimetry (DSC), surface tension measurements, and both steady-state and time-resolved fluorescence spectroscopy. Betaine increased the thermal stability of wild type HLL and protected this enzyme from unfolding by the chaotrope, guanidine...

article

Interactions among Membrane and Soluble Components of the Flagellar Export Apparatus of Salmonella ^†

Keng Zhu, Bertha González-Pedrajo, Robert M. Macnab

Biochemistry > 2002 > 41 > 30 > 9516 - 9524

article

Impact of the tryptophan residues of Humicola lanuginosa lipase on its thermal stability

Keng Zhu, Arimatti Jutila, Esa K.J. Tuominen, Shamkant Anant Patkar, more

Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular Enzymology > 2001 > 1547 > 2 > 329-338

Thermal stability of wild type Humicola lanuginosa lipase (wt HLL) and its two mutants, W89L and the single Trp mutant W89m (W117F, W221H, and W260H), were compared. Differential scanning calorimetry revealed unfolding of HLL at T _d =74.4 ^o C whereas for W89L and W89m this endotherm was decreased to 68.6 and 62 ^o C, respectively, demonstrating significant contribution of the...

article

Detergent-Induced Conformational Changes of Humicola lanuginosa Lipase Studied by Fluorescence Spectroscopy

Arimatti Jutila, Keng Zhu, Shamkant Anant Patkar, Jesper Vind, more

Biophysical Journal > 2000 > 78 > 3 > 1634-1642

Detergent (pentaoxyethylene octyl ether, C₈E₅)-induced conformational changes of Humicola lanuginosa lipase (HLL) were investigated by stationary and time-resolved fluorescence intensity and anisotropy measurements. Activation of HLL is characterized by opening of a surface loop (the “lid”) residing directly over the enzyme active site. The interaction of HLL with C₈E₅ increases fluorescence intensities,...

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Keywords

HUMICOLA LANUGINOSA LIPASE (2)
CALORIMETRY (1)
CROSSLINKING (1)
DEGENERATIVE DISC DISEASE (1)
DIFFERENTIAL SCANNING CALORIMETRY (1)
DSC (1)
FLUORESCENCE SPECTROSCOPY (1)
HYDRATION (1)
PROTEIN (1)
SPINAL DISC (1)
SURFACE TENSION (1)
THERMAL STABILITY (1)
TIME-RESOLVED FLUORESCENCE SPECTROSCOPY (1)
TRIACETIN (1)
TRYPTOPHAN (1)
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Elsevier (5)
ACS (2)
Springer (1)
Wiley (1)

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Search results for: Keng Zhu

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