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Regions of the Torpedo marmorata acetylcholine receptor (AChR) α-subunit involved in the binding of acetylcholine were probed with two different covalent ligands. The sulfhydryl-directed affinity reagent 4-(N-maleimido)phenyltrimethylammonium iodide labeled a single α-subunit cyanogen bromide fragment on the reduced AChR which was identified as α 179-207. The novel photoaffinity ligand p-(N,N-dimethylamino)-benzenediazonium...
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