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The atomic force microscope overestimates lateral dimensions and underestimates heights of nanometer size objects such as proteins and nucleic acids. This has made researchers cautious of AFM measurements, even though there is no other technique capable of measuring topography with sub-nanometer precision. Nevertheless, several approaches for determining the stoichiometry of protein and protein-DNA...
The replication initiator protein RepD recruits the Bacillus PcrA helicase directly onto the (−) strand of the plasmid replication origin oriD. The 5′-phosphate group at the nick is essential for loading, suggesting that it is the RepD covalently linked to the 5′-phosphate group at the nick that loads the helicase onto the oriD. The products of the unwinding reaction were visualised by atomic force...
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