Kirk R. Henne

article

A comparison of substrate dynamics in human CYP2E1 and CYP2A6

John P. Harrelson, Kirk R. Henne, Darwin O.V. Alonso, Sidney D. Nelson

Biochemical and Biophysical Research Communications > 2007 > 352 > 4 > 843-849

Considering the dynamic nature of CYPs, methods that reveal information about substrate and enzyme dynamics are necessary to generate predictive models. To compare substrate dynamics in CYP2E1 and CYP2A6, intramolecular isotope effect experiments were conducted, using deuterium labeled substrates: o-xylene, m-xylene, p-xylene, 2,6-dimethylnaphthalene, and 4,4′-dimethylbiphenyl. Competitive intermolecular...

article

Metabolic activation of the nontricyclic antidepressant trazodone to electrophilic quinone-imine and epoxide intermediates in human liver microsomes and recombinant P4503A4

Amit S. Kalgutkar, Kirk R. Henne, Mary E. Lame, Alfin D.N. Vaz, more

Chemico-Biological Interactions > 2005 > 155 > 1-2 > 10-20

Therapy with the antidepressant trazodone has been associated with several cases of idiosyncratic hepatotoxicity. While the mechanism of hepatotoxicity remains unknown, it is possible that reactive metabolites of trazodone play a causative role. Studies were initiated to determine whether trazodone undergoes bioactivation in human liver microsomes to electrophilic intermediates. LC/MS/MS analysis...

article

Bioactivation of 4-Ipomeanol by CYP4B1: Adduct Characterization and Evidence for an Enedial Intermediate

Brian R. Baer, Allan E. Rettie, Kirk R. Henne

Chemical Research in Toxicology > 2005 > 18 > 5 > 855 - 864

article

Covalent Heme Binding to CYP4B1 via Glu310 and a Carbocation Porphyrin Intermediate ^†

Yi-Min Zheng, Brian R. Baer, M. Byron Kneller, Kirk R. Henne, more

Biochemistry > 2003 > 42 > 15 > 4601 - 4606

article

Genotyping and site-directed mutagenesis of a cytochrome P450 meander Pro-X-Arg motif critical to CYP4B1 catalysis

Y.i-Min Zheng, Kirk R. Henne, Patrick Charmley, Richard B. Kim, more

Toxicology and Applied Pharmacology > 2003 > 186 > 2 > 119-126

CYP4B1 isoforms from rodents and other common laboratory animals are involved in the bioactivation of a range of protoxins, including 2-aminofluorene, 4-ipomeanol, and valproic acid. However, an earlier study provided evidence for a human allele encoding a nonfunctional CYP4B1 enzyme due to a Pro427Ser transversion in the meander region of the protein. In the present study, the CYP4B1 gene from several...

article

Covalent Linkage of Prosthetic Heme to CYP4 Family P450 Enzymes ^†

Kirk R. Henne, Kent L. Kunze, Yi-Min Zheng, Peter Christmas, more

Biochemistry > 2001 > 40 > 43 > 12925 - 12931

article

Active Site Characteristics of CYP4B1 Probed with Aromatic Ligands ^†

Kirk R. Henne, Michael B. Fisher, Krishna R. Iyer, Dieter H. Lang, more

Biochemistry > 2001 > 40 > 29 > 8597 - 8605

article

Chiral phase analysis of warfarin enantiomers in patient plasma in relation to CYP2C9 genotype

Kirk R Henne, Andrea Gaedigk, Ganesh Gupta, J.Steven Leeder, more

Journal of Chromatography B: Biomedical Sciences and Applications > 1998 > 710 > 1-2 > 143-148

A direct chiral-phase high-performance liquid chromatographic method for measuring the ratio of S-warfarin/R-warfarin in patient plasma is described. Plasma samples are first extracted using solid-phase C ₁₈ extraction columns, and the concentrated extracts analyzed using an (R,R) Whelk-O 1 column with a mobile phase of 0.5% glacial acetic acid in acetonitrile. The resulting chromatography...

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Search results for: Kirk R. Henne

A comparison of substrate dynamics in human CYP2E1 and CYP2A6

Metabolic activation of the nontricyclic antidepressant trazodone to electrophilic quinone-imine and epoxide intermediates in human liver microsomes and recombinant P4503A4

Bioactivation of 4-Ipomeanol by CYP4B1: Adduct Characterization and Evidence for an Enedial Intermediate

Covalent Heme Binding to CYP4B1 via Glu310 and a Carbocation Porphyrin Intermediate ^†

Genotyping and site-directed mutagenesis of a cytochrome P450 meander Pro-X-Arg motif critical to CYP4B1 catalysis

Covalent Linkage of Prosthetic Heme to CYP4 Family P450 Enzymes ^†

Active Site Characteristics of CYP4B1 Probed with Aromatic Ligands ^†

Chiral phase analysis of warfarin enantiomers in patient plasma in relation to CYP2C9 genotype

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