Search results for: Ke-He Ruan

Items from 1 to 20 out of 39 results

article

The key residue within the second extracellular loop of human EP3 involved in selectively turning down PGE2- and retaining PGE1-mediated signaling in live cells

Hironari Akasaka, Natasha Thaliachery, Xianghai Zheng, Marissa Blumenthal, more

Archives of Biochemistry and Biophysics > 2017 > 616 > C > 20-29

Key residues and binding mechanisms of PGE₁ and PGE₂ on prostanoid receptors are poorly understood due to the lack of X-ray structures for the receptors. We constructed a human EP3 (hEP3) model through integrative homology modeling using the X-ray structure of the β₂-adrenergic receptor transmembrane domain and NMR structures of the thromboxane A2 receptor extracellular loops. PGE₁ and PGE₂ docking...

article

Identification of the two-phase mechanism of arachidonic acid regulating inflammatory prostaglandin E2 biosynthesis by targeting COX-2 and mPGES-1

Hironari Akasaka, Ke-He Ruan

Archives of Biochemistry and Biophysics > 2016 > 603 > C > 29-37

Through linking inducible cyclooxygenase (COX)-2 with microsomal prostaglandin E2 (PGE₂) synthase-1 (mPGES-1), a Single-Chain Enzyme Complex (SCEC, COX-2-10aa-mPGES-1) was engineered to mimic a specific inflammatory PGE₂ biosynthesis from omega-6 fatty acid, arachidonic acid (AA), by eliminating involvements of non-inducible COX-1 and other PGE₂ synthases. Using the SCEC, we characterized coupling...

article

A TRANSGENIC MOUSE MODEL EXPRESSING A NOVEL SINGLE-CHAIN ENZYME COMPLEX OF CYCLOOXYGENASE AND PROSTACYCLIN SYNTHASE EXHIBITS ROBUST RESISTANCE TO HEART DISEASE AND VASCULAR ASSAULT

Emma Murdoch, Hironari Akasaka, Ke-He Ruan

Journal of the American College of Cardiology > 2016 > 67 > 13(S) > 2288

article

IDENTIFICATION OF THE TWO-PHASE MECHANISM OF ENDOGENOUS OMEGA-6 FATTY ACID, ARACHIDONIC ACID, REGULATING VASCULAR INFLAMMATION BY TARGETING CYCLOOXYGENASE-2 AND MICROSOMAL PROSTAGLANDIN E2 SYNTHASE-1

Hironari Akasaka, Ke-He Ruan

Journal of the American College of Cardiology > 2016 > 67 > 13(S) > 2318

article

Elevated prostacyclin biosynthesis in mice impacts memory and anxiety-like behavior

Craig Vollert, Odochi Ohia, Hironari Akasaka, Casey Berridge, more

Behavioural Brain Research > 2014 > 258 > Complete > 138-144

Prostacyclin is an endogenous lipid metabolite with properties of vasodilation and anti-platelet aggregation. While the effects of prostacyclin on the vascular protection have been well-documented, the role of this eicosanoid in the central nervous system has not been extensively studied. Recently, a transgenic mouse containing a hybrid enzyme, of cyclooxygenase-1 linked to prostacyclin synthase,...

article

Resistant to thrombosis, induced stroke and heart arrest by incorporation of a single gene of PGI2-synthesizing COX-1-PGIS in vivo: Implication against human heart disease

Ke-He Ruan, Anita J. Mohite, Shui-Ping So

International Journal of Cardiology > 2013 > 168 > 3 > 2960-2961

article

COX‐2‐10aa‐PGIS Gene Therapy Improves Erectile Function in Rats after Cavernous Nerve Injury

Haocheng Lin, Jiuhong Yuan, Ke‐He Ruan, Wenli Yang, more

The Journal of Sexual Medicine > 10 > 6 > 1476 - 1487

Introduction.Erectile dysfunction (ED) is a very common complication after radical prostatectomy. COX‐2‐10aa‐PGIS is a newly engineered protein with COX‐2 and prostacyclin synthase activities that converts arachidonic acid directly to prostacyclin (prostaglandin I2 [PGI2]). PGI2 is a potent smooth muscle relaxant. Aim.The purpose of this study was to explore the effect and mechanism of COX‐2‐10aa‐PGIS...

article

Establishing novel prostacyclin-synthesizing cells with therapeutic potential against heart diseases

Ke-He Ruan, Anita Mohite, Shui-Ping So, Cheng-Huai Ruan

International Journal of Cardiology > 2013 > 163 > 2 > 163-169

For decades, there have been many ongoing attempts to use prostaglandin I ₂ (PGI ₂ ) to treat heart diseases, such as pulmonary arterial hypertension. However, the short half life of PGI ₂ has limited the therapeutic impact potential.Here, we have engineered a novel adipose tissue-derived cell that constantly produces PGI _2, through transfecting of an engineered cDNA...

article

Engineered endothelial progenitor cells that overexpress prostacyclin protect vascular cells

Qi Liu, Yutao Xi, Toya Terry, Shui‐Ping So, more

Journal of Cellular Physiology > 227 > 7 > 2907 - 2916

Prostacyclin (PGI2) is a potent vasodilator and important mediator of vascular homeostasis; however, its clinical use is limited because of its short (<2‐min) half‐life. Thus, we hypothesize that the use of engineered endothelial progenitor cells (EPCs) that constitutively secrete high levels of PGI2 may overcome this limitation of PGI2 therapy. A cDNA encoding COX‐1‐10aa‐PGIS, which links human...

article

A profile of NSAID-targeted arachidonic acid metabolisms in human embryonic stem cells (hESCs): Implication of the negative effects of NSAIDs on heart tissue regeneration

Annirudha Chillar, Shui-Ping So, Cheng-Huai Ruan, Harnath Shelat, more

International Journal of Cardiology > 2011 > 150 > 3 > 253-259

An emerging technology using human embryonic stem cells (hESCs) to regenerate infarcted heart tissue has been underdeveloped. However, because non-steroidal anti-inflammatory drugs (NSAIDs), such as aspirin, are taken during the infarction, it becomes critical to know whether the NSAIDs have negative impacts on heart tissue regeneration when using hESCs.Mass spectrometry (LC/MS/MS) and high performance...

article

759 ADIPOSE DERIVED STEM CELLS WITH COX1-10AA-PGIS GENE IMPROVE ERECTILE FUNCTION IN RATS WITH BILATERAL CAVERNOUS NERVE CRUSH INJURY

Haocheng Lin, Jiuhong Yuan, Ke-he Ruan, Yutian Dai, more

The Journal of Urology > 2011 > 185 > 4(S) > e305

article

Novel Mechanism of the Vascular Protector Prostacyclin: Regulating MicroRNA Expression

Anita Mohite, Annirudha Chillar, Shui-Ping So, Vanessa Cervantes, more

Biochemistry > 2011 > 50 > 10 > 1691 - 1699

article

Inducible COX-2 dominates over COX-1 in prostacyclin biosynthesis: Mechanisms of COX-2 inhibitor risk to heart disease

Cheng-Huai Ruan, Shui-Ping So, Ke-He Ruan

Life Sciences > 2011 > 88 > 1-2 > 24-30

Our aim is to understand the molecular mechanisms of the selective nonsteroidal anti-inflammatory drugs (NSAID), cyclooxygenase-2 (COX-2) inhibitors', higher “priority” to reduce synthesis of the vascular protector, prostacyclin (PGI2), compared to that of nonselective NSAIDs.COX-1 or COX-2 was co-expressed with PGI2 synthase (PGIS) in COS-7 cells. The Km and initial velocity (½t Vmax) of the coupling...

article

Structural and Functional Analysis of the C-Terminus of Gαq in Complex with the Human Thromboxane A ₂ Receptor Provides Evidence of Constitutive Activity

Annirudha Chillar, Jiaxin Wu, Vanessa Cervantes, Ke-He Ruan

Biochemistry > 2010 > 49 > 30 > 6365 - 6374

article

Ligand-Specific Conformation Determines Agonist Activation and Antagonist Blockade in Purified Human Thromboxane A2 Receptor ^†

Ke-He Ruan, Vanessa Cervantes, Jiaxin Wu

Biochemistry > 2009 > 48 > 14 > 3157 - 3165

article

Large-scale expression, purification, and characterization of an engineered prostacyclin-synthesizing enzyme with therapeutic potential

Ke-He Ruan, Shui-Ping So, Hanjing Wu, Vanessa Cervantes

Archives of Biochemistry and Biophysics > 2008 > 480 > 1 > 41-50

Recently, we reported that a novel hybrid enzyme (TriCat enzyme), engineered by linking human cyclooxygenase-2 (COX-2) with prostacyclin (PGI ₂ ) synthase (PGIS) together through a transmembrane domain, was able to directly integrate the triple catalytic (TripCat) functions of COX-2 and PGIS and effectively convert arachidonic acid (AA) into the vascular protector, PGI ₂ [K.H. Ruan,...

article

Characterization of the prostaglandin H2 mimic: Binding to the purified human thromboxane A2 receptor in solution

Ke-He Ruan, Cori Wijaya, Vanessa Cervantes, Jiaxin Wu

Archives of Biochemistry and Biophysics > 2008 > 477 > 2 > 396-403

For decades, the binding of prostaglandin H ₂ (PGH ₂ ) to multiple target proteins of unrelated protein structures which mediate diverse biological functions has remained a real mystery in the field of eicosanoid biology. Here, we report that the structure of a PGH ₂ mimic, U46619, bound to the purified human TP, was determined and compared with that of its conformation bound...

article

Involvement of non-conserved residues important for PGE2 binding to the constrained EP3 eLP2 using NMR and site-directed mutagenesis

Annirudha Chillar, Jiaxin Wu, Shui-Ping So, Ke-He Ruan

FEBS Letters > 2008 > 582 > 19 > 2863-2868

A peptide constrained to a conformation of second extracellular loop of human prostaglandin-E ₂ (PGE ₂ ) receptor subtype3 (hEP3) was synthesized. The contacts between the peptide residues at S211 and R214, and PGE ₂ were first identified by NMR spectroscopy. The results were used as a guide for site-directed mutagenesis of the hEP3 protein. The S211L and R214L mutants expressed...

article

A Simple, Quick, and High-Yield Preparation of the Human Thromboxane A ₂ Receptor in Full Size for Structural Studies ^†

Ke-He Ruan, Vanessa Cervantes, Jiaxin Wu

Biochemistry > 2008 > 47 > 26 > 6819 - 6826

article

SUPERENZYME GENE THERAPY IMPROVED PENILE REHABILITATION IN RATS AFTER CAVERNOUS NERVE INJURY THROUGH ANTI-HYPOXIA AND ANTI-APOPTOTIC MECHANISM

Jiuhong Yuan, O Lenaine Westney, Ke-he Ruan, Run Wang

The Journal of Urology > 2008 > 179 > 4 Supplement > 277

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Keywords

CYCLOOXYGENASE (5)
PROSTACYCLIN (5)
NMR (4)
PROSTAGLANDIN (3)
PROSTAGLANDIN E2 (3)
SYNTHETIC PEPTIDE (3)
THROMBOXANE A 2 RECEPTOR (2)
ARACHIDONIC ACID (2)
COX-2 INHIBITOR (2)
CYTOCHROME P450 (2)
G PROTEIN-COUPLING RECEPTOR (2)
PROSTACYCLIN SYNTHASE (2)
PROSTAGLANDIN H2 (2)
PROSTAGLANDIN I2 (2)
THROMBOXANE A2 RECEPTOR (2)
THROMBOXANE A 2 (1)
ANTI-INFLAMMATION (1)
ANTI-THROMBOSIS (1)
ANXIETY (1)
ARACHIDONIC ACID (AA) (1)
BEHAVIOR (1)
CARDIAC ARREST (1)
CAVERNOUS NERVE INJURY (1)
COX-2 (1)
COX2‐10AA‐PGIS (1)
CYCLOOXYGENASE-1 (COX-1) (1)
CYCLOOXYGENASE-2 (1)
EICOSANOID (1)
ENDOMETRIAL CANCER (1)
ENDOMETRIOSIS (1)
EP SELECTIVITY (1)
EP3 RECEPTOR (1)
ERECTILE DYSFUNCTION (1)
EXTRACELLULAR LOOP 2 (1)
G PROTEIN (1)
G PROTEIN-COUPLED RECEPTOR (1)
G PROTEIN-COUPLED RECEPTOR (GPCR) (1)
GPCR (1)
GS PROTEIN (1)
HERBS (1)
HUMAN EMBRYONIC STEM CELLS (1)
MEMORY (1)
MICROSOMAL PROSTAGLANDIN E2 SYNTHASE (1)
MINIGENE (1)
NMR SPECTROSCOPY (1)
NSAIDS (1)
NUCLEAR MAGNETIC RESONANCE (1)
PENILE REHABILITATION (1)
PGI2 SYNTHASE (PGIS) AND FRET (1)
POLYUNSATURATED FATTY ACID (1)
PROSTACYCLIN OR PROSTAGLANDIN I2 (PGI2) (1)
PROSTACYCLIN RECEPTOR (1)
PROSTAGLANDIN E1 (1)
PROSTAGLANDIN I2 SYNTHASE (1)
PROSTANOID RECEPTORS (1)
PROSTANOID SYNTHASE (1)
PROTEIN ENGINEERING (1)
RADICAL PROSTATECTOMY (1)
RECEPTOR REGULATION (1)
SMOOTH MUSCLE CELLS (1)
SOLUTION STRUCTURE (1)
STROKE (1)
THROMBOXANE A2 (1)
THROMBOXANE A2 SYNTHASE (1)
TRADITIONAL MEDICINE (1)
TRANSGENIC MICE (1)
U44069 (1)
U46619 (1)
VASCULAR PROTECTOR (1)
more

Data set

Elsevier (27)
ACS (10)
Wiley (2)

Journal

Archives of Biochemistry and Biophysics (15)
Biochemistry (10)
International Journal of Cardiology (3)
The Journal of Urology (3)
Journal of the American College of Cardiology (2)
Life Sciences (2)
Behavioural Brain Research (1)
FEBS Letters (1)
Journal of Cellular Physiology (1)
The Journal of Sexual Medicine (1)
more

INFONA - science communication portal

Search results for: Ke-He Ruan

The key residue within the second extracellular loop of human EP3 involved in selectively turning down PGE2- and retaining PGE1-mediated signaling in live cells

Identification of the two-phase mechanism of arachidonic acid regulating inflammatory prostaglandin E2 biosynthesis by targeting COX-2 and mPGES-1

A TRANSGENIC MOUSE MODEL EXPRESSING A NOVEL SINGLE-CHAIN ENZYME COMPLEX OF CYCLOOXYGENASE AND PROSTACYCLIN SYNTHASE EXHIBITS ROBUST RESISTANCE TO HEART DISEASE AND VASCULAR ASSAULT

IDENTIFICATION OF THE TWO-PHASE MECHANISM OF ENDOGENOUS OMEGA-6 FATTY ACID, ARACHIDONIC ACID, REGULATING VASCULAR INFLAMMATION BY TARGETING CYCLOOXYGENASE-2 AND MICROSOMAL PROSTAGLANDIN E2 SYNTHASE-1

Elevated prostacyclin biosynthesis in mice impacts memory and anxiety-like behavior

Resistant to thrombosis, induced stroke and heart arrest by incorporation of a single gene of PGI2-synthesizing COX-1-PGIS in vivo: Implication against human heart disease

COX‐2‐10aa‐PGIS Gene Therapy Improves Erectile Function in Rats after Cavernous Nerve Injury

Establishing novel prostacyclin-synthesizing cells with therapeutic potential against heart diseases

Engineered endothelial progenitor cells that overexpress prostacyclin protect vascular cells

A profile of NSAID-targeted arachidonic acid metabolisms in human embryonic stem cells (hESCs): Implication of the negative effects of NSAIDs on heart tissue regeneration

759 ADIPOSE DERIVED STEM CELLS WITH COX1-10AA-PGIS GENE IMPROVE ERECTILE FUNCTION IN RATS WITH BILATERAL CAVERNOUS NERVE CRUSH INJURY

Novel Mechanism of the Vascular Protector Prostacyclin: Regulating MicroRNA Expression

Inducible COX-2 dominates over COX-1 in prostacyclin biosynthesis: Mechanisms of COX-2 inhibitor risk to heart disease

Structural and Functional Analysis of the C-Terminus of Gαq in Complex with the Human Thromboxane A ₂ Receptor Provides Evidence of Constitutive Activity

Ligand-Specific Conformation Determines Agonist Activation and Antagonist Blockade in Purified Human Thromboxane A2 Receptor ^†

Large-scale expression, purification, and characterization of an engineered prostacyclin-synthesizing enzyme with therapeutic potential

Characterization of the prostaglandin H2 mimic: Binding to the purified human thromboxane A2 receptor in solution

Involvement of non-conserved residues important for PGE2 binding to the constrained EP3 eLP2 using NMR and site-directed mutagenesis

A Simple, Quick, and High-Yield Preparation of the Human Thromboxane A ₂ Receptor in Full Size for Structural Studies ^†

SUPERENZYME GENE THERAPY IMPROVED PENILE REHABILITATION IN RATS AFTER CAVERNOUS NERVE INJURY THROUGH ANTI-HYPOXIA AND ANTI-APOPTOTIC MECHANISM

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