Search results for: Mathieu Cinier

Items from 1 to 8 out of 8 results

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Nanofitins targeting heat shock protein 110: An innovative immunotherapeutic modality in cancer

Guillaume Marcion, François Hermetet, Fabrice Neiers, Burhan Uyanik, more

International Journal of Cancer > 148 > 12 > 3019 - 3031

The presence of an inactivating heat shock protein 110 (HSP110) mutation in colorectal cancers has been correlated with an excellent prognosis and with the ability of HSP110 to favor the formation of tolerogenic (M2‐like) macrophages. These clinical and experimental results suggest a potentially powerful new strategy against colorectal cancer: the inhibition of HSP110. In this work, as an alternative...

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Regiospecific radiolabelling of Nanofitin on Ni magnetic beads with [18F]FBEM and in vivo PET studies

Sylvestre Dammicco, Marine Goux, Christian Lemaire, Guillaume Becker, more

Nuclear Medicine and Biology > 2017 > 51 > C > 33-39

Nanofitins are low molecular weight, single chain and cysteine-free protein scaffolds able to selectively bind a defined biological target. They derive from Sac7d bacterial protein family and are highly stable over a wide range of pH (0–13) and temperature (Tm ~80°C). Their extreme stability, low cost of production and high tolerability for chemical coupling make Nanofitins a very interesting alternative...

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In vivo phosphorylation of a peptide tag for protein purification

Marine Goux, Amina Fateh, Alain Defontaine, Mathieu Cinier, more

Biotechnology Letters > 2016 > 38 > 5 > 767-772

Objectives To design a new system for the in vivo phosphorylation of proteins in Escherichia coli using the co-expression of the α-subunit of casein kinase II (CKIIα) and a target protein, (Nanofitin) fused with a phosphorylatable tag. Results The level of the co-expressed CKIIα was controlled by the arabinose promoter and optimal phosphorylation was obtained with 2 % (w/v) arabinose as inductor...

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Mo1687 Oral Delivery of a New Class of Non-Antibody Protein Scaffold Nanofitins Targeting TNF-Alpha Shows a Strong Preventive and Curative Anti-Inflammatory Effect in Models of Inflammatory Bowel Diseases

Christel Rousseaux, Mathieu Cinier, Magali Zeisser Labouebe, Joao V. Rodrigues, more

Gastroenterology > 2015 > 148 > 4(S1) > S-685

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Engineering of a phosphorylatable tag for specific protein binding on zirconium phosphonate based microarrays

Mathieu Cinier, Marc Petit, Frédéric Pecorari, Daniel R. Talham, more

JBIC Journal of Biological Inorganic Chemistry > 2012 > 17 > 3 > 399-407

A phosphorylatable tag was designed and fused at the C-terminal end of proteins, which allowed efficient and oriented immobilization of capture proteins on glass substrates coated with a zirconium phosphonate monolayer. The concept is demonstrated using Nanofitin directed against lysozyme. This peptide tag (DSDSSSEDE) contains four serines in an acidic environment, which favored its in vitro phosphorylation...

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Assembly of multifunctional phi29 pRNA nanoparticles for specific delivery of siRNA and other therapeutics to targeted cells

Yi Shu, Mathieu Cinier, Dan Shu, Peixuan Guo

Methods > 2011 > 54 > 2 > 204-214

Recent advances in RNA nanotechnology have led to the emergence of a new field and brought vitality to the area of therapeutics [P. Guo, The emerging field of RNA nanotechnology, Nat. Nanotechnol., 2010]. Due to the complementary nature of the four nucleotides and its special catalytic activity, RNA can be manipulated with simplicity characteristic of DNA, while possessing versatile structure and...