Fluorescence lifetime (FLT) measurements in the long-wavelength red and near-infrared (NIR) range are expected to improve the reliability and robustness of fluorescence-based detection. This review provides a summary of suitable classes of red and NIR luminescent reporters for use in FLT-based applications.
We describe the spectral properties of an amine-reactive, pH-sensitive, long-wavelength ratiometric fluorescent label having a pK a in the physiological pH range. The label exhibits its main absorption and emission in the near-infrared (NIR) region. On deprotonation, a blue shift of the excitation maximum is observed. Importantly, both the protonated and deprotonated forms of the label are...
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