Search results for: Anika C. Jahns

Items from 1 to 13 out of 13 results

article

Transcriptomic analysis of Propionibacterium acnes biofilms in vitro

Anika C. Jahns, Hinnerk Eilers, Oleg A. Alexeyev

Anaerobe > 2016 > 42 > C > 111-118

Propionibacterium acnes is a well-known commensal of the human skin connected to acne vulgaris and joint infections. It is extensively studied in planktonic cultures in the laboratory settings but occurs naturally in biofilms. In this study we have developed an in vitro biofilm model of P. acnes and studied growth features, matrix composition, matrix penetration by fluorescent-labeled antibiotics...

article

Microbial colonization of normal skin: Direct visualization of 194 skin biopsies

Anika C. Jahns, Oleg A. Alexeyev

Anaerobe > 2016 > 38 > C > 47-49

Recent genetic studies have suggested the presence of numerous microbial species on and in the skin. We characterised microbial colonization of a large collection of skin biopsies from 194 healthy subjects by fluorescence assay. Forty per cent of all biopsies did not show any evidence for microbial colonization. Propionibacterium acnes was the sole predominant bacterial species in both sebaceous and...

article

Immobilization of active lipase B from Candida antarctica on the surface of polyhydroxyalkanoate inclusions

Anika C. Jahns, Bernd H. A. Rehm

Biotechnology Letters > 2015 > 37 > 4 > 831-835

Polyhydroxyalkanoate (PHA) beads, recombinantly produced in Escherichia coli , were functionalized to display lipase B from Candida antarctica as translational protein fusion. The respective beads were characterized in respect to protein content, functionality, long term storage capacity and re-usability. The direct fusion of the PHA synthase, PhaC, to lipase B yielded...

article

Three dimensional distribution of Propionibacterium acnes biofilms in human skin

Anika C. Jahns, Oleg A. Alexeyev

Experimental Dermatology > 23 > 9 > 687 - 689

Propionibacterium acnes is regarded as a common member of the human skin microbiota, often occurring in biofilms. Little is known about the size of bacterial biofilms in hair follicles as a few sections of biopsy tissue are routinely evaluated. Transversal sectioning provides a better opportunity for histological analyses of hair follicles which can be followed through the different morphological...

article

Microbiology of hidradenitis suppurativa (acne inversa): a histological study of 27 patients

Anika C. Jahns, Hassan Killasli, Daniel Nosek, Bertil Lundskog, more

APMIS > 122 > 9 > 804 - 809

Hidradenitis suppurativa (acne inverse) (HS) is a chronic skin disease primarily affecting hair follicles. The aetiology of HS is unknown, but infection is believed to play some role. This retrospective study investigated the microbial colonization directly in skin appendices in HS skin samples. Archival samples from 27 patients with HS were screened by immunofluorescence labelling with monoclonal...

article

Microbiology of folliculitis: a histological study of 39 cases

Anika C. Jahns, Bertil Lundskog, Johanna Berg, Rebecca Jonsson, more

APMIS > 122 > 1 > 25 - 32

Folliculitis is a common inflammatory skin syndrome. Several microbial organisms have been put forward as causative agents, but few studies visualized microbes directly in inflamed hair follicles. This retrospective study investigated bacterial and fungal colonization of inflamed hair follicles in patients with clinically diagnosed non‐infectious folliculitis. Skin biopsies from 39 folliculitis patients...

article

Simultaneous visualization of Propionibacterium acnes and Propionibacterium granulosum with immunofluorescence and fluorescence in situ hybridization

Anika C. Jahns, Cristina Oprica, Ismini Vassilaki, Irina Golovleva, more

Anaerobe > 2013 > 23 > Complete > 48-54

Propionibacterium acnes (P. acnes) and Propionibacterium granulosum (P. granulosum) are common skin colonizers that are implicated as possible contributing factors in acne vulgaris development. We have established direct visualization tools for the simultaneous detection of these closely related species with immunofluorescence assay and fluorescence in situ hybridization (FISH). As proof of principle,...

article

Spatial distribution of bacterial–fungal communities in facial skin

Anika C. Jahns, Irina Golovleva, Ruth H. Palmer, Oleg A. Alexeyev

Journal of Dermatological Science > 2013 > 70 > 1 > 71-73

article

No link between rosacea and Propionibacterium acnes

Anika C. Jahns, Bertil Lundskog, Ida Dahlberg, Natalia Curiche Tamayo, more

APMIS > 120 > 11 > 922 - 925

Rosacea is a common skin disease in adults affecting mainly the facial skin. Although inflammation appears to play a pathogenic role in rosacea, initiating factors are largely unknown. Microbial involvement in the development of rosacea has been suggested previously. We aimed to visualize Propionibacterium acnes in the skin compartments of rosacea patients. Facial skin biopsies from 82 rosacea patients...