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Tetrodotoxin (TTX) is a high toxic small molecular neurotoxin. Haptenic vaccine for TTX was investigated and the carrier proteins were compared. TTX was conjugated to Tachypleus tridentatus hemocyanin (TTH) and tetanus toxoid (TT) via formaldehyde to form the artificial antigen TTX-TTH and TTX-TT. BALB/c mice were immunized with the artificial antigen, the TTX-specific antibody response were detected...
The polyclonal antibodies specific for tetrodotoxin (TTX) were prepared from mice and their capacity of neutralizing TTX was investigated so as to explore the possibility of developing TTX antitoxin. Haptenic TTX was conjugated to Tachypleus tridentatus hemocyanin (TTH) chemically to form artificial antigen TTX–TTH. BALB/c mice were immunized with TTX–TTH and ascites were induced by intraperitoneal...
An electrochemical immunosensor for the direct determination of paraoxon has been developed based on the biocomposites of gold nanoparticles loaded with paraoxon antibodies. The biocomposites are immobilized on the glassy carbon electrode (GCE) using Nafion membrane. On the immunosensor prepared paraoxon shows well-shaped CV with reduction and oxidation peaks located -0.08 and -0.03 mV versus SCE,...
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