Effects of high-voltage pulsed electric field (PEF) on native or thermal denatured enzyme activities were studied. When PEF was applied to various native enzymes, 105–120% of initial enzyme activities were observed after PEF treatment. It was suggested that an activation of enzyme would be possible by PEF treatment. We attempted a refolding of thermal denatured enzyme by using PEF. When PEF was applied...
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