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It was found that human erythrocytes underwent hemolysis when incubated with peroxynitrite at 37°C under air. The extent of hemolysis increased with increasing peroxynitrite concentration and decreasing hematocrit. The peroxynitrite-induced hemolysis was suppressed only partially by a radical scavenging antioxidant such as uric acid and Trolox, a water-soluble vitamin E analogue, but reduced glutathione,...
The damaging effect of perfusion of hydrophilic radical generating azo compound on the liver of normal and vitamin E-deficient rats and its inhibition by antioxidants were studied in order to increase understanding of the action of free radicals on biological tissues. The hepatic damage was evaluated from the release of cytosolic enzymes such as glutamic oxaloacetic transaminase and glutamic pyruvic...
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