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We describe a photoswitchable DNA‐based dimeric dye that visibly changes fluorescence from green to blue upon UV irradiation. A novel bis‐alkyne‐dependent [2+2+2] cycloaddition is proposed as a mechanism for the color change in air. The photoinduced structural switching results in spatial separation of stacked pyrene units, thereby causing selective loss of the excimer emission. We demonstrate and...
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