Search results for: Mei-Hwa Lee

Items from 1 to 20 out of 31 results

article

A multichannel system integrating molecularly imprinted conductive polymers for ultrasensitive voltammetric determination of four steroid hormones in urine

Mei-Hwa Lee, James L. Thomas, Wei-Chiun Liu, Zheng-Xiang Zhang, more

Microchimica Acta > 2019 > 186 > 11 > 1-11

This work reports on a modularized electrochemical method for the determination of the hormones cortisol, progesterone, testosterone and 17β-estradiol in urine. These hormones were employed as templates when generating molecular imprints from aniline and metanilic acid by electropolymerization on the surface of screen-printed electrodes. The electrically conductive imprint was characterized by SEM,...

article

Gold-decorated magnetic nanoparticles modified with hairpin-shaped DNA for fluorometric discrimination of single-base mismatch DNA

Mei-Hwa Lee, Ching-Chich Leu, Cheng-Chih Lin, Yu-Fan Tseng, more

Microchimica Acta > 2019 > 186 > 2 > 1-8

The authors describe the use of gold-decorated magnetic nanoparticles (Au/MNPs) in discriminating DNA sequences with a single-base (guanine) mismatch. The Au/MNPs were characterized through dynamic light scattering, X-ray diffraction, superconducting quantum interference device, and UV/visible spectroscopy. They were then conjugated to a probe oligomer consisting of a hairpin-shaped DNA sequence carrying...

article

Epitope recognition of peptide-imprinted polymers for Regenerating protein 1 (REG1)

Mei-Hwa Lee, James L. Thomas, Chun-Lin Liao, Stipo Jurcevic, more

Separation and Purification Technology > 2018 > 192 > C > 213-219

Molecularly imprinted polymers (MIPs) were developed to replace natural antibodies with a cost-effective and durable synthetic material. Molecular imprinting of proteins conventionally utilizes the whole protein as the template, which is complex (as many different epitopes may be imprinted) and expensive. In this work, seven peptides (13–18 amino acids) were synthesized and used as templates for the...

article

Enhanced supercapacitor performance using molecular self-assembling polyaniline onto carbon nanoparticles

Po-Hsin Wang, Tzong-Liu Wang, Wen-Churng Lin, Hung-Yin Lin, more

Journal of Electroanalytical Chemistry > 2018 > 810 > C > 145-153

This work studied the effect of self-doped polyaniline (SPANI)-assembled carbon nanoparticles (CNPs) on a supercapacitor. SPANI was in situ polymerised and assembled on CBs by mixing the monomers o-aminobenzenesulphonic acid (SAN) and aniline (AN) with an initiator and CNPs. SAN acted as a self-doped monomer and a surfactant. SPANI-modified CNPs used as an electrode material would improve the performance...

article

Polymers imprinted with three REG1B peptides for electrochemical determination of Regenerating Protein 1B, a urinary biomarker for pancreatic ductal adenocarcinoma

Mei-Hwa Lee, James L. Thomas, Chun-Lin Liao, Stipo Jurcevic, more

Microchimica Acta > 2017 > 184 > 6 > 1773-1780

Three peptides (each containing 13–18 amino acids) were synthesized and used as templates for molecular imprinting and epitope recognition of the Regenerating Protein 1B (REG1B), which is one of the urinary biomarkers for pancreatic ductal adenocarcinoma (PDAC). Poly(ethylene-co-vinyl alcohol)s were employed as the host for molecular imprinting of the peptides. Following their preparation, the molecularly...

article

Electrochemical sensing of nuclear matrix protein 22 in urine with molecularly imprinted poly(ethylene-co-vinyl alcohol) coated zinc oxide nanorod arrays for clinical studies of bladder cancer diagnosis

Mei-Hwa Lee, James L. Thomas, Yu-Chia Chang, Yuh-Shyan Tsai, more

Biosensors and Bioelectronics > 2016 > 79 > C > 789-795

In 1996 and 2000, the US Food and Drug Administration (FDA) approved the use of Nuclear matrix protein 22 (NMP22) as a monitoring tool for predicting the recurrence/clearing of bladder cancer, and for screening undiagnosed individuals who have symptoms of, or are at risk for, that disease. The fabrication of electrodes for sensing NMP22 and their integration with a portable potentiostat in a homecare...

article

Recognition of Rhodobacter sphaeroides by microcontact-imprinted poly(ethylene-co-vinyl alcohol)

Mei-Hwa Lee, James L. Thomas, Ming-Huan Li, Ching-Ping Shih, more

Colloids and Surfaces B: Biointerfaces > 2015 > 135 > Complete > 394-399

The immobilization of cells or microorganisms is important for bioseparations, in bioreactors producing cellular metabolites, and as receptors for biosensing. Cell-imprinted polymers (CIPs) have been shown to have cavities with complementary shapes and also high affinities for the template cells or microorganisms. However, the effects of binding to CIPs on gene expression are only beginning to be...

article

Integrated potentiostat for electrochemical sensing of urinary 3-hydroxyanthranilic acid with molecularly imprinted poly(ethylene-co-vinyl alcohol)

Chun-Yueh Huang, Danny O'Hare, I-Jen Chao, Hung-Wei Wei, more

Biosensors and Bioelectronics > 2015 > 67 > C > 208-213

Changing demographics, the rise of personalized medicine and increased identification of biomarkers for diagnosis and management of chronic disease have increased the demand for portable bioanalytical instrumentation and point-of-care. The recent development of molecularly imprinted polymers enables production of low cost and highly stable sensing chips; however, the commercially available and full...

chapter

A Novel Supercritical CO₂ Synthesis of Amorphous Hydrous Zirconia Nanoparticles, and Their Calcination to Zirconia

Mei‐Hwa Lee, Hung‐Yin Lin, James L. Thomas

Progress in Nanotechnology > 129 - 135

A novel synthesis of amorphous hydrous zirconia nanoparticles was performed in a supercritical carbon dioxide reverse microemulsion, converting a high concentration of a very inexpensive starting material into a product that was then calcined to yield monoclinic zirconia nanoparticles. The amorphous hydrous zirconia precursor particles were obtained by simply adding a precipitating agent to [Zrˆ)|/perfluoropolyether/scC0...

article

Preparation of curcumin microemulsions with food-grade soybean oil/lecithin and their cytotoxicity on the HepG2 cell line

Chuan-Chuan Lin, Hung-Yin Lin, Ming-Hung Chi, Chin-Min Shen, more

Food Chemistry > 2014 > 154 > Complete > 282-290

The choice of surfactants and cosurfactants for preparation of oral formulation in microemulsions is limited. In this report, a curcumin-encapsulated phospholipids-based microemulsion (ME) using food-grade ingredients soybean oil and soybean lecithin to replace ethyl oleate and purified lecithin from our previous study was established and compared. The results indicated soybean oil is superior to...

article

Optical sensing of urinary melatonin with molecularly imprinted poly(ethylene-co-vinyl alcohol) coated zinc oxide nanorod arrays

Mei-Hwa Lee, James L. Thomas, Yi-Li Chen, Chien-Fu Lin, more

Biosensors and Bioelectronics > 2013 > 47 > Complete > 56-61

In this work, aligned zinc oxide (ZnO) nanorods were selectively hydrothermally grown on acetate-seeded spots on a gold substrate; the nanorods had an average length and diameter of 1.7μm and 240nm, respectively. Melatonin was imprinted into poly(ethylene-co-vinyl alcohol), EVAL, which was coated onto ZnO nanorod arrays. The ZnO nanorods not only increased the surface area for sensing target molecules,...

article

Synthesis of Zirconia with Nanoporous Structure by a Supercritical Carbon Dioxide Microemulsion Route

Mei‐Hwa Lee, Hung‐Yin Lin, James L. Thomas

International Journal of Applied Ceramic Technology > 7 > 6 > 874 - 880

Supercritical carbon dioxide (scCO₂) fluid as a green solvent for processing can markedly reduce the use of organic solvents. Nanoporous clusters of zirconia (ZrO₂) were synthesized in a [Zr⁴⁺](aq)/scCO₂ microemulsion using hydrazine solution as the precipitating agent, and the resulting nanostructures (surface area, morphology) under different reaction temperatures and pressures were determined by...

article

The complete replacement of antibodies by protein-imprinted poly(ethylene-co-vinyl alcohol) in sandwich fluoroimmunoassays

Mei-Hwa Lee, James L. Thomas, Yun-Chao Chen, Wei-Ti Chin, more

Microchimica Acta > 2013 > 180 > 15-16 > 1393-1399

The replacement of antibodies by molecularly imprinted polymers (MIPs) has been investigated for many decades. However, indirect protocols (including natural primary and secondary antibodies) are still utilized to evaluate the ability of MIP thin films to recognize target molecules. MIPs can be prepared as either a thin film or as particles, and cavities that are complementary to the template can...

chapter

Design of a signal processing circuit for quartz crystal microbalance biosensors

Shih-Chang Chang, I-Jen Chao, Bin-Da Liu, Chun-Yueh Huang, more

2011 9th IEEE International Conference on ASIC > 180 - 183

2011 IEEE 9th International Conference on ASIC (ASICON 2011)

In this paper, we design a signal processing circuit for quartz crystal microbalance (QCM) biosensors. The proposed signal processing circuit, which consists of two oscillators, a mixer, a low-pass filter, can be used to measure small amount of QCM biosensors' frequency variation at a fundamental resonance frequency. Based on the principle of heterodyne technique, the QCM biosensors' frequency variation...

article

Comparison of the Antioxidant and Transmembrane Permeative Activities of the Different Polygonum cuspidatum Extracts in Phospholipid-Based Microemulsions

Mei-Hwa Lee, Li Kao, Chuan-Chuan Lin

Journal of Agricultural and Food Chemistry > 2011 > 59 > 17 > 9135 - 9141

article

Sensing of Digestive Proteins in Saliva with a Molecularly Imprinted Poly(ethylene- co -vinyl alcohol) Thin Film Coated Quartz Crystal Microbalance Sensor

Mei-Hwa Lee, James L. Thomas, Hong-Yi Tseng, Wei-Che Lin, more

ACS Applied Materials & Interfaces > 2011 > 3 > 8 > 3064 - 3071

article

Hydrothermal synthesis and characterization of mesoporous zinc selenide agglomerates by nitrogen bubble templates

Hung-Yin Lin, Jyh-Ding Wei, Chia-Chih Ou, Jin-Wei Lu, more

Journal of Alloys and Compounds > 2011 > 509 > 25 > 7009-7015

Mesoporous coral-like and litchi-like zinc selenide agglomerates were successfully synthesized with sodium selenite and zinc acetate dihydrate as precursors by adding hydrazine hydrate using the hydrothermal method. The experimental parameters were varied and hard agglomerates of small nanoparticles were observed. Increasing amounts of hydrazine hydrate were added to control the pH values of the reaction...

chapter

A microfluidic and potentiostatic sensor integrated with neopterin-imprinted poly(ethylene-c0-vinyl alcohol) based electrode

Chen-Hsin Hsieh, Hung-Yin Lin, Chien-Fu Lin, Hann-Huei Tsai, more

2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems > 575 - 578

2011 IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

Neopterin is a catabolic product of guanosine triphosphate (GTP), a purine nucleotide. In human biological fluids such as urine, neopterin concentrations indicate the activation of cellular immune activation under the control of T helper cells. High neopterin concentrations in body fluids such as serum or urine also indicate the activation of cellular immunity, which is associated with the reaction...

chapter

A microfluidic and potentiostatic sensor integrated with neopterin-imprinted poly(ethylene-co-vinyl alcohol) based electrode

Chen-Hsin Hsieh, Hung-Yin Lin, Chien-Fu Lin, Hann-Huei Tsai, more

2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems > 711 - 714

2011 IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

article

Synthesis of Magnetic Molecularly Imprinted Poly(ethylene- co -vinyl alcohol) Nanoparticles and Their Uses in the Extraction and Sensing of Target Molecules in Urine

Mei-Hwa Lee, James L. Thomas, Min-Hsien Ho, Ching Yuan, more

ACS Applied Materials & Interfaces > 2010 > 2 > 6 > 1729 - 1736

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Keywords

POLY(ETHYLENE-CO-VINYL ALCOHOL) (8)
CYCLIC VOLTAMMETRY (4)
MOLECULAR IMPRINTING (4)
URINE (4)
BIOSENSORS (3)
MOLECULARLY IMPRINTING (3)
CREATININE (2)
CURCUMIN (2)
CURRENT MEASUREMENT (2)
ELECTROCHEMICAL SENSING (2)
ELECTROCHEMICAL SENSORS (2)
ELECTRODES (2)
LECITHIN (2)
MICROEMULSIONS (2)
MOLECULALY IMPRINITNG (2)
NANOPARTICLES (2)
NEOPTERIN (2)
PLASTICS (2)
POLY(ETHYLENE-CO-ETHYLENE ALCOHOL) (2)
SALIVARY PROTEINS (2)
VOLTAMMETRY (CHEMICAL ANALYSIS) (2)
(-)EPIGALLOCATECHIN GALLATE (1)
3-HYDROXYANTHRANILIC ACID (1)
AFM (1)
ALBUMIN (1)
AMORPHOUS HYDROUS ZIRCONIA NANOPARTICLES (1)
ANTIAGING (1)
ARTIFICIAL ANTIBODY (1)
BIOMEMBRANES (1)
CAFFEINE (1)
CALCINATION TEMPERATURE (1)
CANCER (1)
CARBON NANOPARTICLE (1)
CHROMATOGRAPHY (1)
COMPOSITE PARTICLES (1)
CONVERTERS (1)
CURRENT DENSITY (1)
CYTOTOXICITY (1)
DNA SEQUENCES (1)
DOPAMINE BIOSENSOR (1)
DOPAMINE SENSING (1)
ELECTRIC POTENTIAL (1)
ELECTRIC VARIABLES MEASUREMENT (1)
ELECTROCHEMICAL DOPAMINE BIOSENSOR (1)
ELECTROCHEMICAL URINALYSIS (1)
EMULSION DROPLET SIZE (1)
ENCAPSULATION (1)
EPITOPE RECOGNITION (1)
ESCA (1)
EVAL (1)
EVAPORATION (1)
EXTRACTION (1)
FREQUENCY CONVERSION (1)
FREQUENCY MEASUREMENT (1)
GENE EXPRESSION (1)
GOLD-DECORATED MAGNETIC NANOPARTICLES (1)
HERBAL EXTRACTS (1)
HEXACYANOFERRATE (1)
HIGH-PRECISION INSTRUMENTS (1)
HOME-CARE SYSTEM (1)
HOMECARE APPLICATIONS (1)
HORMONES (1)
HYDROTHERMAL SYNTHESIS (1)
INSULATING THIN FILMS (1)
INTEGRATED POTENTIOSTAT (1)
ISOFLAVONES (1)
LINEARITY (1)
LIQUID CHROMATOGRAPHY (1)
LOW NOISE MEASUREMENTS (1)
LYSOZYME (1)
MASS 400 MG (1)
MASS 72 MG (1)
MEAN PARTICLE SIZE (1)
MELATONIN (1)
MEMBRANE (1)
MEMBRANES (1)
MESOPOROUS MATERIAL (1)
MICROCONTACT-IMPRINTING (1)
MICROEMULSION (1)
MICROPARTICLES (1)
MIP (1)
MODULATION (1)
MOLECULAR BIOPHYSICS (1)
MOLECULAR IMPRINTED POLYMER MEMBRANE (1)
MOLECULAR SELF-ASSEMBLING (1)
MOLECULARLY IMPRINTED POLY(ETHYLENE-CO-VINYL-ALCOHOL) RECOGNITION ELEMENT (1)
MOLECULARLY IMPRINTED POLYMERIC COATED ELECTRODE (1)
MOLECULARLY IMPRINTED POLYMERIC ELECTRODE (1)
MOLECULARLY IMPRINTED POLYMERS (1)
MULTICHANNEL POTENTIOSTAT (1)
NITROGENASE (1)
NUCLEAR MATRIX PROTEIN 22 (1)
ORGANIC COMPOUNDS (1)
OXIDATION (1)
OXIDATION POTENTIAL (1)
PATIENT TREATMENT (1)
PDAC (1)
PEPTIDE IMPRINTED POLYMER (1)
PEPTIDE IMPRINTING (1)
PERMEABILITY (1)
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Elsevier (14)
ACS (5)
ieee (5)
Springer (5)
Wiley (2)

Journal

Biosensors and Bioelectronics (5)
Microchimica Acta (4)
Food Chemistry (3)
ACS Applied Materials & Interfaces (2)
Journal of Agricultural and Food Chemistry (2)
Analytical and Bioanalytical Chemistry (1)
Chemical Engineering Science (1)
Colloids and Surfaces B: Biointerfaces (1)
Desalination (1)
International Journal of Applied Ceramic Technology (1)
Journal of Alloys and Compounds (1)
Journal of Electroanalytical Chemistry (1)
Langmuir (1)
Separation and Purification Technology (1)
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INFONA - science communication portal

Search results for: Mei-Hwa Lee

A multichannel system integrating molecularly imprinted conductive polymers for ultrasensitive voltammetric determination of four steroid hormones in urine

Gold-decorated magnetic nanoparticles modified with hairpin-shaped DNA for fluorometric discrimination of single-base mismatch DNA

Epitope recognition of peptide-imprinted polymers for Regenerating protein 1 (REG1)

Enhanced supercapacitor performance using molecular self-assembling polyaniline onto carbon nanoparticles

Polymers imprinted with three REG1B peptides for electrochemical determination of Regenerating Protein 1B, a urinary biomarker for pancreatic ductal adenocarcinoma

Electrochemical sensing of nuclear matrix protein 22 in urine with molecularly imprinted poly(ethylene-co-vinyl alcohol) coated zinc oxide nanorod arrays for clinical studies of bladder cancer diagnosis

Recognition of Rhodobacter sphaeroides by microcontact-imprinted poly(ethylene-co-vinyl alcohol)

Integrated potentiostat for electrochemical sensing of urinary 3-hydroxyanthranilic acid with molecularly imprinted poly(ethylene-co-vinyl alcohol)

A Novel Supercritical CO₂ Synthesis of Amorphous Hydrous Zirconia Nanoparticles, and Their Calcination to Zirconia

Preparation of curcumin microemulsions with food-grade soybean oil/lecithin and their cytotoxicity on the HepG2 cell line

Optical sensing of urinary melatonin with molecularly imprinted poly(ethylene-co-vinyl alcohol) coated zinc oxide nanorod arrays

Synthesis of Zirconia with Nanoporous Structure by a Supercritical Carbon Dioxide Microemulsion Route

The complete replacement of antibodies by protein-imprinted poly(ethylene-co-vinyl alcohol) in sandwich fluoroimmunoassays

Design of a signal processing circuit for quartz crystal microbalance biosensors

Comparison of the Antioxidant and Transmembrane Permeative Activities of the Different Polygonum cuspidatum Extracts in Phospholipid-Based Microemulsions

Sensing of Digestive Proteins in Saliva with a Molecularly Imprinted Poly(ethylene- co -vinyl alcohol) Thin Film Coated Quartz Crystal Microbalance Sensor

Hydrothermal synthesis and characterization of mesoporous zinc selenide agglomerates by nitrogen bubble templates

A microfluidic and potentiostatic sensor integrated with neopterin-imprinted poly(ethylene-c0-vinyl alcohol) based electrode

A microfluidic and potentiostatic sensor integrated with neopterin-imprinted poly(ethylene-co-vinyl alcohol) based electrode

Synthesis of Magnetic Molecularly Imprinted Poly(ethylene- co -vinyl alcohol) Nanoparticles and Their Uses in the Extraction and Sensing of Target Molecules in Urine

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