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Proteins carrying sulfated glycans (i.e., sulfated glycoproteins) are known to be associated with diseases, such as cancer, cystic fibrosis, and osteoarthritis. Sulfated glycoproteins, however, have not been isolated or characterized from complex biological samples due to lack of appropriate tools for their enrichment. Here, we describe a method to identify and characterize sulfated glycoproteins...
We examined the influence of nucleobases, nucleosides, nucleotides, and their analogs on rat cerebellar Purkinje cells in primary culture and found that the number of cultured Purkinje cells was greatly increased by the nucleobase adenine. Purkinje cells were cultured for 13 days in vitro in the presence of various reagents, and the resulting cell numbers were counted. As a result, the nucleobase...
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