Search results for: Gabriella Leo

Items from 1 to 17 out of 17 results

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Regulating levels of the neuromodulator d‐serine in human brain: structural insight into pLG72 and d‐amino acid oxidase interaction

Leila Birolo, Silvia Sacchi, Giovanni Smaldone, Gianluca Molla, more

The FEBS Journal > 283 > 18 > 3353 - 3370

The human flavoenzyme d‐amino acid oxidase (hDAAO) degrades the NMDA‐receptor modulator d‐serine in the brain. Although hDAAO has been extensively characterized, little is known about its main modulator pLG72, a small protein encoded by the primate‐specific gene G72 that has been associated with schizophrenia susceptibility. pLG72 interacts with neosynthesized hDAAO, promoting its inactivation and...

article

Ultraviolet laser‐induced cross‐linking in peptides

Gabriella Leo, Carlo Altucci, Sandrine Bourgoin‐Voillard, Alfredo M. Gravagnuolo, more

Rapid Communications in Mass Spectrometry > 27 > 14 > 1660 - 1668

RATIONALEThe aim of this study was to demonstrate, and to characterize by high‐resolution mass spectrometry that it is possible to preferentially induce covalent cross‐links in peptides by using high‐energy femtosecond ultraviolet (UV) laser pulses. The cross‐link is readily formed only when aromatic amino acids are present in the peptide sequence. METHODSThree peptides, xenopsin, angiotensin I, and...

article

A family GH51 α-l-arabinofuranosidase from Pleurotus ostreatus : identification, recombinant expression and characterization

Antonella Amore, Angela Amoresano, Leila Birolo, Bernard Henrissat, more

Applied Microbiology and Biotechnology > 2012 > 94 > 4 > 995-1006

An α-l-arabinofuranosidase produced by Pleurotus ostreatus (PoAbf) during solid state fermentation on tomato pomace was identified and the corresponding gene and cDNA were cloned and sequenced. Molecular analysis showed that the poabf gene carries 26 exons interrupted by 25 introns and has an open reading frame encoding a protein of 646 amino acid residues, including a signal peptide of 20 amino acid...

article

Fungal solid state fermentation on agro-industrial wastes for acid wastewater decolorization in a continuous flow packed-bed bioreactor

Donata Iandolo, Antonella Amore, Leila Birolo, Gabriella Leo, more

Bioresource Technology > 2011 > 102 > 16 > 7603-7607

This study was aimed at developing a process of solid state fermentation (SSF) with the fungi Pleurotus ostreatus and Trametes versicolor on apple processing residues for wastewater decolorization. Both fungi were able to colonize apple residues without any addition of nutrients, material support or water. P. ostreatus produced the highest levels of laccases (up to 9Ug ⁻¹ of dry matter) and...

article

Deamidation at Asparagine and Glutamine As a Major Modification upon Deterioration/Aging of Proteinaceous Binders in Mural Paintings

Gabriella Leo, Ilaria Bonaduce, Alessia Andreotti, Gennaro Marino, more

Analytical Chemistry > 2011 > 83 > 6 > 2056 - 2064

article

PHK from phenol hydroxylase of Pseudomonas sp. OX1. Insight into the role of an accessory protein in bacterial multicomponent monooxygenases

Viviana Izzo, Gabriella Leo, Roberta Scognamiglio, Luca Troncone, more

Archives of Biochemistry and Biophysics > 2011 > 505 > 1 > 48-59

Bacterial multicomponent monooxygenases (BMMs) are members of a wide family of diiron enzymes that use molecular oxygen to hydroxylate a variety of aromatic compounds. The presence of genes encoding for accessory proteins not involved in catalysis and whose role is still elusive, is a common feature of the gene clusters of several BMMs, including phenol hydroxylases and several soluble methane monooxygenases...

article

Stoichiometry and Topology of the Complex of the Endogenous ATP Synthase Inhibitor Protein IF ₁ with Calmodulin

Daniela Pagnozzi, Leila Birolo, Gabriella Leo, Stefania Contessi, more

Biochemistry > 2010 > 49 > 35 > 7542 - 7552

article

Identification of a new member of Pleurotus ostreatus laccase family from mature fruiting body

Vincenzo Lettera, Alessandra Piscitelli, Gabriella Leo, Leila Birolo, more

Fungal Biology > 2010 > 114 > 9 > 724-730

Laccases (benzenediol:oxygen oxidoreductases, EC 1.10.3.2) are blue multicopper oxidases, catalyzing the oxidation of an array of aromatic substrates concomitantly with the reduction of molecular oxygen to water. Most of the known laccases have fungal or plant origins, although few laccases have been also identified in bacteria and insects. Most of the fungal laccases reported thus far are extra-cellular...

article

Identification of ΔNp63α Protein Interactions by Mass Spectrometry

Angela Amoresano, Antonella Di Costanzo, Gabriella Leo, Ferdinando Di Cunto, more

Journal of Proteome Research > 2010 > 9 > 4 > 2042 - 2048

article

Single-Layer InAs Quantum Dots for High-Performance Planar Photodetectors Near 1.3 $\mu\hbox{m}$

Anna Persano, Bahram Nabet, Marc Currie, Annalisa Convertino, more

IEEE Transactions on Electron Devices > 2010 > 57 > 6 > 1237 - 1242

The potential of InAs quantum-dot (QD) photodetectors for room-temperature high-speed operation at wavelengths near 1.3 μm is evaluated. Specifically, planar metal-semiconductor-metal structures on GaAs substrates containing one absorption layer of self-assembled InAs QDs embedded in Ga(In)As matrices are fabricated, characterized, and analyzed. Light absorption, optically generated carrier transport,...

article

Proteomic strategies for the identification of proteinaceous binders in paintings

Gabriella Leo, Laura Cartechini, Piero Pucci, Antonio Sgamellotti, more

Analytical and Bioanalytical Chemistry > 2009 > 395 > 7 > 2269-2280

The identification of proteinaceous components in paintings remains a challenging task for several reasons. In addition to the minute amount of sample available, complex and variable chemical composition of the paints themselves, possible simultaneous presence of several binders and contaminants, and degradation of the original materials due to aging and pollution are complicating factors. We proposed...

article

Effect of shape and surface chemistry of TiO2 colloidal nanocrystals on the organic vapor absorption capacity of TiO2/PMMA composite

Annalisa Convertino, Gabriella Leo, Marinella Striccoli, Gaetano Di Marco, more

Polymer > 2008 > 49 > 25 > 5526-5532

The organic vapor absorption capacity of poly(methyl metacrylate) (PMMA), filled with oleic acid (OLEA) capped TiO ₂ nanocrystals (NCs) with curved shape, rod-like and spherical, is studied. The NC shape combined with the nature of the capping molecules can be used to enhance or reduce the PMMA ability to absorb different solvent molecules in a controlled way. Indeed, the arrangement of the...

article

Shell thickness dependence of exciton trapping in colloidal core/shell nanorods

Arianna Cretì, Mauro Lomascolo, Gabriella Leo, Liberato Manna, more

Journal of Luminescence > 2008 > 128 > 3 > 361-365

We quantitatively investigated, by time-resolved photoluminescence (PL) spectroscopy, the shell thickness dependence of exciton trapping and its effects on the PL quantum yield (QY) in colloidal CdSe/CdS/ZnS core/shell quantum rods. The defects passivation, due to a thin shell (0.6 monolayer), leads to a 2 times reduction of the trapping from both emitting and high-energy excited states, thus explaining...

article

Thin films of TiO2 nanocrystals with controlled shape and surface coating for surface plasmon resonance alcohol vapour sensing

Maria Grazia Manera, Pantaleo Davide Cozzoli, Gabriella Leo, Maria Lucia Curri, more

Sensors & Actuators: B. Chemical > 2007 > 126 > 2 > 562-572

This work explores the potential of thin films composed of shape-controlled colloidal TiO ₂ nanocrystals in optical gas sensing applications. To this purpose, an optical characterization of nanocrystal thin films has been performed by means of UV–vis spectroscopy and surface plasmon resonance (SPR) technique. In particular, this technique has been used as optical transduction method to test...

article

Growth interruption to tune the emission of InAs quantum dots embedded in InGaAs matrix in the long wavelength region

Annalisa Convertino, Luciana Cerri, Gabriella Leo, Sesto Viticoli

Journal of Crystal Growth > 2004 > 261 > 4 > 458-465

We investigate the effects of combining growth interruption (GI) and InGaAs strain reducing matrix at low In content on the optical properties of Stanski-Krastanow InAs QDs grown by molecular beam epitaxy. Atomic force microscopy and Photoluminescence (PL) measurements were carried out on three series of uncapped and capped samples grown in different conditions: (a) low arsenic pressure (As BEP=4x10...

article

ZnO Nanocrystals by a Non-hydrolytic Route: Synthesis and Characterization

P. Davide Cozzoli, M. Lucia Curri, Angela Agostiano, Gabriella Leo, more

The Journal of Physical Chemistry B > 2003 > 107 > 20 > 4756 - 4762

article

Laser irradiation effects on the resistance of SmS films

Ferdinando de Tomasi, Maria Rita Perrone, Maria Lucia Protopapa, Gabriella Leo

Thin Solid Films > 2002 > 413 > 1-2 > 171-176

Samarium monosulfide (SmS) films deposited by flash evaporation to realise pressure sensors have been processed by ultraviolet laser radiation at 308 nm to modulate their electrical resistance. The laser irradiation effects on the resistance of SmS films have been investigated at different laser fluences. It is shown that the SmS film resistance can be significantly modulated, even at fluences lower...

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