Enzymatic bioactivation of an otherwise non‐reactive species into a reactive one can lead to significant health problems in vivo. Bioactivation of benzo[a]pyrene (BP) produces known cancer causing agents, most notably anti‐benzo[a]pyrene‐r‐7,t‐8‐dihydrodiol‐t‐9,10‐epoxide (anti‐BPDE) that has been shown to attack DNA at specific genomic sites termed hotspots. Bioactivation of BP resulting in DNA damage...
A simple electrochemical assay to monitor the alginate penetration ability of a series of antimicrobial peptides (AMP) containing unnatural amino acids is reported. Alginate, a component of P. aeruginosa biofilms, was used to modify pyrolytic graphite (PG) electrodes using well-established layer-by-layer (LbL) protocols, followed by exposure to AMP at varying concentrations for increasing lengths...
This chapter contains sections titled:
Electrochemical and Electrochemiluminescent Arrays
DNA/Enzyme Biocolloids for LC‐MS Toxicity Screening
Alternative Arrays and Other Novel Approaches
Summary and Future Outlook
Simultaneous optical and voltammetric detection of bioactivated genotoxicity is reported for the first time employing ultrathin films of DNA, model metabolic enzymes, and electrochemiluminescence (ECL) generating metallopolymer [Ru(bpy) 2 PVP 10 ] 2+ on pyrolytic graphite (PG) electrodes. Cytochrome P450 cam and myoglobin were used as model monoxygenase enzymes to mimic...
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