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Commercial samples of Coomassie Brilliant Blue G-250 (CBB) were not pure enough to give reliable results when used as indicator of amine content in biological material. The polar and apolar impurities produce unacceptable biases in the results. Counter current chromatography (CCC) was used to purify significant amounts of CBB. The liquid system heptane/1-butanol/water 2:3:4 (v/v) was appropriate to...
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