Search results for: Jiri Plachy

Items from 1 to 18 out of 18 results

article

„DLOUHÉ“ 20. STOLETÍ PLK. KARLA KUBÍČKA

Jiŕí Plachý

Vojenská história > 2019 > 23 > 4 > 29 – 52

In the form of biographical probe, the study follows the stories of an individual, the Czech officer Karel Kubíček (1896–1964) against the backdrop of historical events of the “turbulent” 20th century. As a passionate patriot, he returned from the World War 1 as the officer of CS Legions in Russia, serving as a professional soldier in the CS Army for two interwar decades. However, for personal reasons,...

article

Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines

Jitka Mucksová, Jiří Plachý, Ondřej Staněk, Jiří Hejnar, more

Veterinary Research > 2017 > 48 > 1 > 1-14

Systems of antigen delivery into antigen-presenting cells represent an important novel strategy in chicken vaccine development. In this study, we verified the ability of Rous sarcoma virus (RSV) antigens fused with streptavidin to be targeted by specific biotinylated monoclonal antibody (anti-CD205) into dendritic cells and induce virus-specific protective immunity. The method was tested in four congenic...

article

B‐cell inhibition by cross‐linking CD79b is superior to B‐cell depletion with anti‐CD20 antibodies in treating murine collagen‐induced arthritis

Hilke Brühl, Josef Cihak, Yvonne Talke, Manuel Rodriguez Gomez, more

European Journal of Immunology > 45 > 3 > 705 - 715

Depletion of B cells with the anti‐CD20 antibody rituximab is an established therapy for rheumatoid arthritis. However, rituximab has only moderate efficacy, most likely due to insufficient depletion of B cells in lymphoid organs and expansion of pathogenic B cells. We found that an antibody against mouse CD79b profoundly blocks B‐cell proliferation induced via the B‐cell receptor, CD40, CD180, and...

article

K činnosti některých negativistických stran na Podkarpatské Rusi v roce 1938

Jiří Plachý

Slovanský přehled: historická revue pro dějiny střední, východní a jihovýchodní... > 2012 > 98 > 1-2 > 117-136

The study concentrates on the political scene in Carpathian Ruthenia during the months of 1938 and the preceding Vienna Award as viewed by the political press service of the Provincial Office for Carpathian Ruthenia in Uzhhorod. The Czechoslovak authorities and security forces paid primary attention to the Heinlein's Carpathian German Party and the Unified Hungarian Party. They were also concerned...

article

Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling

Lenka Doubravska, Michaela Krausova, Dietmar Gradl, Martina Vojtechova, more

Cellular Signalling > 2011 > 23 > 5 > 837-848

The Wnt family of proteins is a group of extracellular signalling molecules that regulate cell-fate decisions in developing and adult tissues. It is presumed that all 19 mammalian Wnt family members contain two types of post-translational modification: the covalent attachment of fatty acids at two distinct positions, and the N-glycosylation of multiple asparagines. We examined how these modifications...

article

Generation of two modified mouse alleles of the Hic1 tumor suppressor gene

Vendula Pospichalova, Jolana Tureckova, Bohumil Fafilek, Martina Vojtechova, more

genesis > 49 > 3 > 142 - 151

HIC1 (hypermethylated in cancer 1) is a tumor suppressor gene located on chromosome 17p13.3, a region frequently hypermethylated or deleted in human neoplasias. In mouse, Hic1 is essential for embryonic development and exerts an antitumor role in adult animals. Since Hic1‐deficient mice die perinatally, we generated a conditional Hic1 null allele by flanking the Hic1‐coding region by loxP sites. When...

article

The Chicken Z Chromosome Is Enriched for Genes with Preferential Expression in Ovarian Somatic Cells

Libor Mořkovský, Radka Storchová, Jiří Plachý, Robert Ivánek, more

Journal of Molecular Evolution > 2010 > 70 > 2 > 129-136

Theory predicts that sexually antagonistic mutations will be over- or under-represented on the X and Z chromosomes, depending on their average dominance coefficients. However, as little is known about the dominance coefficients for new mutations, the effect of sexually antagonistic selection is difficult to predict. To elucidate the role of sexually antagonistic selection in the evolution of Z chromosome...

article

The transcription factor EGR1 regulates metastatic potential of v-src transformed sarcoma cells

Vladimír Čermák, Jan Kosla, Jiří Plachý, Kateřina Trejbalová, more

Cellular and Molecular Life Sciences > 2010 > 67 > 20 > 3557-3568

Metastatic spreading of cancer cells is a highly complex process directed primarily by the interplay between tumor microenvironment, cell surface receptors, and actin cytoskeleton dynamics. To advance our understanding of metastatic cancer dissemination, we have developed a model system that is based on two v-src transformed chicken sarcoma cell lines—the highly metastatic parental PR9692 and a non-metastasizing...

article

Československý podíl na spojenecké pomoci při výstavbě jugoslávských jednotek v letech 1944-1945. Příspěvek k československo-jugoslávským vztahům

Jiri Plachy

Slovanský přehled: historická revue pro dějiny střední, východní a jihovýchodní... > 2010 > 96 > 1-2 > 161-167

The opening section of the study briefly sums up certain aspects of the western allies' military aid to the Yugoslav national liberation movement under the leadership of Josip Broz Tito in 1943-1945. Attention is paid to the less known participation of the Czechoslovak foreign resistance in this aid. At least 2,340 Yugoslavian citizens underwent a basic military training with the Reserve Troops of...

article

Chicken C-type lectin-like receptor B-NK, expressed on NK and T cell subsets, binds to a ligand on activated splenocytes

Birgit C. Viertlboeck, Andreas Wortmann, Ramona Schmitt, Jiří Plachý, more

Molecular Immunology > 2008 > 45 > 5 > 1398-1404

B-NK is a C-type lectin with an immunorecptor tyrosine-based inhibition motif, that is located in the vicinity of the chicken MHC and that has been described as a potential chicken NK cell receptor. We have generated an epitope tagged B-NK version for immunization and for biochemical studies. B-NK was expressed as a heavily glycosylated, homodimeric, type II transmembrane protein. With the help of...

article

Development of a green fluorescent protein metastatic-cancer chick-embryo drug-screen model

Vladimir Bobek, Jiri Plachy, Daniela Pinterova, Katarina Kolostova, more

Clinical & Experimental Metastasis > 2004 > 21 > 4 > 347-352

The chick-embryo model has been an important tool to study tumor growth, metastasis, and angiogenesis. However, an imageable model with a genetic fluorescent tag in the growing and spreading cancer cells that is stable over time has not been developed. We report here the development of such an imageable fluorescent chick-embryo metastatic cancer model with the use of green fluorescent protein (GFP)...

article

Deletions in the dna-binding domain of the TP53 gene in v-src-transformed chicken cells

Kateřina Trtková, Jiři Plachý

In Vitro Cellular & Developmental Biology - Animal > 2004 > 40 > 8-9 > 285-292

Summary We have examined the chicken TP53 tumor suppressor gene in v-src-transformed chicken tumor cells by reverse transcriptase-polymerase chain reaction and deoxyribonucleic acid (DNA) sequencing. Initially, we have detected frequent deletions of variable length in both DNA-binding and oligomerization domains of the TP53 in late as well as early in vitro passages of the chicken tumor cell line...

article

v-src oncogene-specific carboxy-terminal peptide is immunoprotective against Rous sarcoma growth in chickens with MHC class I allele B-F12

Andrea Hofmann, Jirı́ Plachy, Lawrence Hunt, Jim Kaufman, more

Vaccine > 2003 > 21 > 32 > 4694-4699

B ¹ ² haplotype of the inbred chicken line CB (B ¹ ² /B ¹ ² ) contains, like the bulk of chicken MHC(B) haplotypes, only a single dominantly expressed class I molecule (B-F). The peptide binding motifs for this major B-F12 molecule in chickens of Rous sarcoma regressor line CB (B ¹ ² /B ¹ ² ) have been determined. Using stringent and relaxed...

article

DNA vaccination against v-src oncogene-induced tumours in congenic chickens

Jiřı́ Plachý, Jiřı́ Hejnar, Kateřina Trtková, Kateřina Trejbalová, more

Vaccine > 2001 > 19 > 31 > 4526-4535

DNA vaccination is particularly efficient for induction of cytotoxic T-lymphocyte (CTL) response. In our experiments, we used MHC(B) congenic chicken lines CB and CC (regressors and progressors of v-src-induced tumours, respectively) and a mutated, non-oncogenic v-src gene construct as the DNA vaccine. A high degree of vaccine protection against oncogenic v-src challenge was achieved in the CB line...

article

Expression of Nonspecific Esterase (NSE) by Thyroid Follicular Epithelium as a Marker for the Target Organ Susceptibility to Immune System Attack

Karel Hala, Alojz Kúbek, Jiří Plachý, Dušan Vašíček

Immunobiology > 2000 > 201 > 5 > 598-610

Spontaneous autoimmune thyroiditis in obese strain (OS) chickens provides an excellent animal model for the study of Hashimoto's autoimmune thyroiditis in humans. The data presented in this paper indicate that nonspecific esterases (NSE) may play a role in or serve as a marker for the target organ susceptibility. Experiments have shown that follicular epithelial cells and interfollicular macrophages...

article

Protective Effects of Type I and Type II Interferons toward Rous Sarcoma Virus-Induced Tumors in Chickens

Jiřı́ Plachý, Kirsten C. Weining, Elisabeth Kremmer, Florian Puehler, more

Virology > 1999 > 256 > 1 > 85-91

Growth of tumors induced by Rous sarcoma virus (RSV) is controlled by alleles at the major histocompatibility complex locus in chickens, indicating that immunological host defense mechanisms play a major role. We show here that the resistance phenotype of CB regressor chickens can be partially reverted by treating the animals with a monoclonal antibody that neutralizes the major serotype of chicken...

article

Inhibition of the Rous Sarcoma Virus Long Terminal Repeat-Driven Transcription byin VitroMethylation: Different Sensitivity in Permissive Chicken Cells versus Mammalian Cells

Jiřı́ Hejnar, Jiřı́ Plachý, Josef Geryk, Ondřej Machoň, more

Virology > 1999 > 255 > 1 > 171-181

Rous sarcoma virus (RSV) enhancer sequences in the long terminal repeat (LTR) have previously been shown to be sensitive to CpG methylation. We report further that the high density methylation of the RSV LTR-driven chloramphenicol acetyltransferase reporter is needed for full transcriptional inhibition in chicken embryo fibroblasts and for suppression of tumorigenicity of the RSV proviral DNA in chickens...

article

Inhibition of the Development of Spontaneous Autoimmune Thyroiditis in the Obese Strain (OS) Chickens byIn VivoTreatment With Anti-CD4 or Anti-CD8 Antibodies

Josef Cihak, Gertrud Hoffmann-Fezer, Michael Wasl, Heidrun Merkle, more

Journal of Autoimmunity > 1998 > 11 > 2 > 119-126

The involvement of CD4 ⁺ and CD8 ⁺ T cells in pathogenesis of spontaneous autoimmune thyroiditis (SAT) in obese strain (OS) chickens has not been studied in depth until now. We depleted CD4 ⁺ or CD8 ⁺ T cells in OS chickens by treatment with murine monoclonal anti-CD4 or anti-CD8 antibodies at 3 day intervals beginning at hatching. The birds were killed at 19–25 days...

Filter options

Publication date

Set your own date range

INFONA - science communication portal

Search results for: Jiri Plachy

Add recipient

Sending message cancelled

Are you sure you want to cancel sending this message?

Send message

Filter options

Publication date

Date range setting

Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.

Content availability

Publication language

Keywords

Data set

Journal

Reporting an error / abuse

Sending the report failed

Accessibility options