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A rapid and sensitive procedure to detectMycoplasma bovisfrom milk samples was developed. Mycoplasma cells were collected by selective binding to a monoclonal antibody, thus separating them from milk proteins and facilitating DNA extraction. Polymerase chain reaction (PCR) using species-specific primers allowed detection of 20 cfu ml −1 in agarose gels and 2 cfu ml −1 on Southern blots...
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