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We developed a simple and effective analysis procedure that includes pretreatment and determination methods for β-N-methylamino-l-alanine (BMAA), a cyanobacterial neurotoxin. BMAA may be produced by all known groups of cyanobacteria living in freshwater as well as marine environments. In this paper, we report a novel determination method for BMAA. A cation-exchange resin was effective for the selective...
The molecular extinction coefficient ε of cylindrospermopsin (CYN) purified by the anion exchange and the normal-phase HPLC procedures was determined to be 9800 at 262nm. This ε is significantly higher than those (ε, 5800–6250) reported previously. In order to determine CYN concentrations in solutions using UV absorption, the ε-value of CYN should be corrected from 5800 to 9800. Further, the [α] ...
A new simply and effective fractionation method for cylindrospermopsin (CYN) analyses was developed. The extract from cells of Cylindrospermopsis raciborskii was resuspended with 0.1M carbonate buffer at pH 10.5, and pass through the double-cartridges column which was consisted of a styrene polymer cartridge and an anion exchange cartridge. CYN and deoxy-CYN were adsorbed with the anion exchange cartridge...
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