To investigate the effects of a six-week plyometric training and creatine supplementation intervention on maximal-intensity and endurance performance in female soccer players during in-season training.Randomized, double-blind, placebo-controlled trial.Young (age 22.9±2.5y) female players with similar training load and competitive background were assigned to a plyometric training group receiving placebo (PLACEBO, n=10), a plyometric training group receiving creatine supplementation (CREATINE, n=10) or a control group receiving placebo without following a plyometric program (CONTROL, n=10). Athletes were evaluated for jumping, maximal and repeated sprinting, endurance and change-of-direction speed performance before and after six weeks of training.After intervention the CONTROL group did not change, whereas both plyometric training groups improved jumps (ES=0.25–0.49), sprint (ES=0.35–0.41), repeated sprinting (ES=0.48–0.55), endurance (ES=0.32–0.34) and change-of-direction speed performance (ES=0.46–0.55). However, the CREATINE group improved more in the jumps and repeated sprinting performance tests than the CONTROL and the PLACEBO groups.Adaptations to plyometric training may be enhanced with creatine supplementation.