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Key points
Using high‐speed videos time‐locked with whole‐animal electrical recordings, simultaneous measurement of behavioural kinematics and field potential parameters of C‐start startle responses allowed for discrimination between short‐latency and long‐latency C‐starts (SLCs vs. LLCs) in larval zebrafish.
Apart from their latencies, SLC kinematics and SLC field potential parameters were intensity...
We achieved a 100-fold improvement in tip-sample stability by stabilizing the tip and the sample in 3D using laser light back-scattered off the apex of an AFM tip and a reference mark in the sample.
The most commonly used optical-trapping assays are coupled to surfaces, yet such assays lack atomic-scale (∼0.1 nm) spatial resolution due to drift between the surface and trap. We used active stabilization techniques to minimize surface motion to 0.1 nm in three dimensions and decrease multiple types of trap laser noise (pointing, intensity, mode, and polarization). As a result, we achieved nearly...
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